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Recombinant Human GLP-1R Fc Chimera Protein, CF

Catalog # 10646-GL | R&D Systems, Inc. a Bio-Techne Brand
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10646-GL-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human GLP-1R protein
Human GLP-1R
(Ala21-Glu139)
Accession # P43220.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21 & Arg24

Predicted Molecular Mass

40 kDa

SDS-PAGE

60-72 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When GLP-1 peptide (Tocris (Catalog # 5374) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human GLP-1 Fc Chimera binds with an ED50 of 2-12 ng/mL.

Scientific Data Images for Recombinant Human GLP-1R Fc Chimera Protein, CF

Recombinant Human GLP-1R Fc Chimera Protein Binding Activity.

When GLP-1 peptide (5374) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human GLP-1R Fc Chimera (Catalog # 10646-GL) binds with an ED50 of 2-12 ng/mL.

Recombinant Human GLP-1R Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human GLP-1R Fc Chimera (Catalog # 10646-GL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-72 kDa and 120-144 kDa, respectively.

Formulation, Preparation and Storage

10646-GL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLP-1R

Glucagon-like Peptide 1 Receptor (GLP-1R) is a member of the Class B or secretin receptor-like family of G protein-coupled receptors (GPCRs) (1, 2). Activation of GLP-1R signaling regulates secretion of insulin from pancreatic beta-cells in a glucose-dependent manner (3). Human GLP-1R consists of an N-terminal, extracellular domain (ECD) containing 6 conserved cysteine residues and a transmembrane domain with seven membrane-spanning alpha-helices, characteristic of GPCRs (2,4). The mature ECD of human GLP-1R shares 92% amino acid (aa) identity with mouse GLP-1R. GLP-1R is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach and it is a receptor for Glucagon-like Peptide, a regulator of insulin secretion by pancreatic beta cells (1, 4, 5). GLP1R agonists, including GLP-1, Boc5, and Cpd1, have been targeted for research and used as type 2 diabetes treatments (5, 6). GLP1R is also expressed in the brain where it is involved in the control of appetite (5).

References

  1. Song, G. et al. (2017) Nature. 546:312.
  2. Hollenstein, K. et al. (2013) Trends Pharmacol Sci. 35:12.
  3. Drucker, D.J. et al. (1987) PNAS. 84 (10):3434.
  4. Donnelly, D. (2012) Br. J. Pharmacol. 166:27.
  5. Simo, R. and Hernandez, C. (2017) Diabetes. 66:1453.
  6. Holst, J.J. (2004) Expert Opinion on Emerging Drugs. 9:155.

Long Name

Glucagon-like Peptide 1 Receptor

Alternate Names

GLP1R

Entrez Gene IDs

2740 (Human); 14652 (Mouse)

Gene Symbol

GLP1R

UniProt

Product Documents for Recombinant Human GLP-1R Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human GLP-1R Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

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