Recombinant Human GDNF GMP Protein, CFGMP

Name: Recombinant Human GDNF GMP Protein, CF
Brand: rnd
SKU: 212-GMP

Catalog # 212-GMP

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Bio-Techne China Co. Ltd.

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Key Product Details

Source:	NS0
Accession #:	P39905
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity, Bioactivity

View Full Technical Details for Recombinant Human GDNF GMP Protein, CF

Product Specifications

Source	Mouse myeloma cell line, NS0-derived human GDNF protein Arg109-Ile211 Manufactured and tested under cGMP guidelines.
Purity	>97%, by SDS-PAGE with silver staining, under reducing conditions.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Arg₁₀₉-Gly-Gln-Arg-Gly-Lys-Asn-Arg-Gly-(Cys)
Predicted Molecular Mass	11.6 kDa (monomer)
Activity	Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED₅₀ for this effect is 2-12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR). The specific activity of recombinant human GDNF is >5.0 x 10⁵ units/mg, which is calibrated against the human GDNF Reference Standard (NIBSC code: 09/266). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) at 1 µg/mL can bind Recombinant Human GDNF with an apparent K_d <1 nM.

Recombinant Human GDNF GMP Protein, CF Scientific Data Examples

	Recombinant Human GDNF GMP Protein Bioactivity GMP-grade Recombinant Human GDNF (Catalog # 212-GMP) stimulates proliferation in the SH-SY5Y human neuroblastoma cell line. The ED₅₀for this effect is 2-12 ng/mL in the presence of Recombinant Human GFRa-1/GDNF Ra-1 Fc Chimera (Catalog # 714-GR).
	Recombinant Human GDNF GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human GDNF (Catalog # 212-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 17 kDa and 33 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

212-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Item	Value
Long Name	Glial Cell line-derived Growth Factor
Entrez Gene IDs	2668 (Human); 25453 (Rat)
Alternate Names	ATF, ATF1, ATF2, Astrocyte-derived trophic factor, GDNF, HFB1-GDNF, HGDNF, HSCR3, glial cell derived neurotrophic factor, glial cell line derived neurotrophic factor, glial cell line-derived neurotrophic factor

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Recombinant Human GDNF GMP Protein, CFGMP

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Recombinant Human GDNF GMP Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: GDNF

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