Recombinant Human Flt-3/Flk-2 Fc Chimera Protein

Name: Recombinant Human Flt-3/Flk-2 Fc Chimera Protein
Brand: R&D Systems
SKU: 368-ST

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 368-ST

Citations (4)

Product Datasheet / COA / SDS

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	AAA18947
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Flt-3/Flk-2 Fc Chimera Protein

Product Specifications for Recombinant Human Flt-3/Flk-2 Fc Chimera Protein

Source

Mouse myeloma cell line, NS0-derived human Flt-3/Flk-2 protein

Human Flt-3 (Asn27-Asn541) Accession # AAA18947	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Asn27

Predicted Molecular Mass

85.5 kDa (monomer)

SDS-PAGE

120 kDa, reducing conditions

Activity

Measured by its ability to inhibit Flt-3 Ligand-induced proliferation of BaF3 mouse pro‑B cells transfected with Flt-3. The ED₅₀ for this effect is 0.01-0.03 µg/mL in the presence of rhFlt-3 Ligand at 2 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

368-ST

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

368-ST/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

Item	Value
References	Rosnet, O. et al. (1996) Acta. Haemato. 95:218. Drexler, H.G. (1996) Leukemia 10:588.
Long Name	fms-like Tyrosine Kinase 3
Entrez Gene IDs	2322 (Human); 14255 (Mouse)
Alternate Names	CD135, CD135 antigen, EC 2.7.10, FL cytokine receptor, FLK2, FLT3 receptor tyrosine kinase, Flk-2, Flt-3, Flt3, Fms-like tyrosine kinase 3, STK-1, STK1EC 2.7.10.1, Stem cell tyrosine kinase 1, Tyrosine-protein kinase receptor FLT3, fetal liver kinase 2, fms-related tyrosine kinase 3, growth factor receptor tyrosine kinase type III

Citations for Recombinant Human Flt-3/Flk-2 Fc Chimera Protein (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Flt-3/Flk-2 Fc Chimera Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Q Jiang et al. (2021), Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolution Cell Reports, 34(4):108670.
PMID: 33503434
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

MA Kinney et al. (2019), A systems biology pipeline identifies regulatory networks for stem cell engineering Nat. Biotechnol., 37(7):810-818.
PMID: 31267104
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Heshmati et al. (2018), The histone chaperone NAP1L3 is required for haematopoietic stem cell maintenance and differentiation Sci Rep, 8(1):11202.
PMID: 30046127
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay

Donahue RE et al. (2000), High levels of lymphoid expression of enhanced green fluorescent protein in nonhuman primates transplanted with cytokine-mobilized peripheral blood CD34(+) cells. Blood, 95(2):445-52.
PMID: 10627448

Recombinant Human Flt-3/Flk-2 Fc Chimera Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Flt-3/Flk-2 Fc Chimera Protein

Formulation, Preparation and Storage

Carrier Free

368-ST

Carrier

368-ST/CF

Carrier Free

Reconstitution Calculator

Background: Flt-3/Flk-2

Citations for Recombinant Human Flt-3/Flk-2 Fc Chimera Protein (4)

Product Documents for Recombinant Human Flt-3/Flk-2 Fc Chimera Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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