Recombinant Human FGFR5/FGFRL1 Protein, CF

Name: Recombinant Human FGFR5/FGFRL1 Protein, CF
Brand: R&D Systems
SKU: 9805-FR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9805-FR

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	Q8N441
Applications:	Bioactivity

View Full Technical Details for Recombinant Human FGFR5/FGFRL1 Protein, CF

Product Specifications for Recombinant Human FGFR5/FGFRL1 Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human FGF R5/FGFRL1 protein Ala25-Pro378, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala25
Predicted Molecular Mass	40 kDa
SDS-PAGE	54-63 kDa, reducing conditions
Activity	Measured by the ability of the immobilized protein to support the adhesion of MG-63 human bone fibroblasts. The ED₅₀ for this effect is 1.5-7.5 μg/mL.

Scientific Data Examples for Recombinant Human FGFR5/FGFRL1 Protein, CF

	Recombinant Human FGFR5/FGFRL1 Protein Binding Activity Recombinant Human FGF R5/FGFRL1 (Catalog # 9805-FR) supports MG-63 human bone Fibroblasts adhesion. The ED₅₀ for this effect is 1.5-7.5 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9805-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: FGFR5/FGFRL1

Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2-6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6). FGF R5 is found in most multicellular animals. It is known to bind to FGF ligands, with high affinity to FGF-3, -4, -8, -10, and -22 and weaker affinity to FGF-2, -5, -17, -18 and -23 (7). The extracellular domain of FGF R5 promotes cell adhesion when mediated by heparin sulfate glycosaminoglycans on the cell surface (8, 9). Comparing to the signaling receptors of FGFR family members (FGF R1-FGF R4), FGF R5 is more similar to the nectins for its adhesion properties and has no effect on cell growth and proliferation (9).

Item	Value
References	Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107. Sleeman, M. et al. (2001) Gene 271:171. Kim, I. et al. (2001) Biochim. Biophys. Acta 1518:152. Antoine, M. et al. (2005) Growth Factors 23:87. Wiedemann, M. et al. (2000) Genomics 69:275. Trueb, B. et al. (2003) J. Biol. Chem. 278:33857. Steinberg, F. et al (2010). J Biol Chem 285:2193. Rieckmann, T. et al (2008) Exp Cell Res 314:1071. Yang, X. et al (2016) International J Molec Med. 38:30.
Long Name	Fibroblast Growth Factor Receptor-like 1
Entrez Gene IDs	53834 (Human); 116701 (Mouse)
Alternate Names	FGF R5, FGF homologous factor receptor, FGF receptor-like protein 1, FGFR-5, FGFR-like protein, FGFR5, FGFRL1, FHFR, Fibroblast growth factor receptor 5, fibroblast growth factor receptor-like 1

Citations for Recombinant Human FGFR5/FGFRL1 Protein, CF

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Recombinant Human FGFR5/FGFRL1 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Recombinant Human FGFR5/FGFRL1 Protein, CF

Scientific Data Examples for Recombinant Human FGFR5/FGFRL1 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: FGFR5/FGFRL1

Citations for Recombinant Human FGFR5/FGFRL1 Protein, CF

Product Documents for Recombinant Human FGFR5/FGFRL1 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected