Recombinant Human FGFR1 alpha (IIIc) His-tag Protein, CF

Name: Recombinant Human FGFR1 alpha (IIIc) His-tag Protein, CF
Brand: R&D Systems
SKU: 11118-FR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11118-FR

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Source:	HEK293
Accession #:	P11362.3
Applications:	Bioactivity

View Full Technical Details for Recombinant Human FGFR1 alpha (IIIc) His-tag Protein, CF

Product Specifications for Recombinant Human FGFR1 alpha (IIIc) His-tag Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human FGFR1 alpha protein Arg22-Glu376, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Arg22
Predicted Molecular Mass	40 kDa
SDS-PAGE	70-85 kDa, under reducing conditions.
Activity	Measured by its binding ability in a functional ELISA. In a Human FGF acidic/FGF1 antibody (Catalog # AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (Catalog # 232-FA), Human FGFR1 alpha (IIIc) His-tag Protein binds with an ED₅₀ of 1.00-5.00 µg/mL.

Scientific Data Examples for Recombinant Human FGFR1 alpha (IIIc) His-tag Protein, CF

	Recombinant Human FGFR1 alpha (IIIc) His-tag Protein Binding Activity. In a Human FGF acidic/FGF1 antibody (AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1 (232-FA), Human FGFR1 alpha (IIIc) His-tag Protein (Catalog # 11118-FR) binds with an ED₅₀ of 1.00‑5.00 µg/mL.
	Recombinant Human FGFR1 alpha (IIIc) His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human FGFR1 alpha (IIIc) His-tag Protein (Catalog # 11118-FR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-85 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11118-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: FGFR1 alpha

Fibroblast growth factor receptor 1 (FGFR1) belongs to a family of type I transmembrane tyrosine kinases which mediate the biological functions of FGFs that are involved in a multitude of physiological and pathological cellular processes (1). The FGFR family is comprised of 4 structurally conserved members (FGFR1-4) all possessing an extracellular domain (ECD) with three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and cytoplasmic split tyrosine-kinase domain (1, 2). The ECD of mature, full-length FGFR1 shares 98% amino acid sequence identity with mouse FGFR1. Alternative splicing generates multiple forms of FGFR1-3, each with unique signaling characteristics (1-3). For FGFR1, alternative splicing of the ECD generates FGFR1A, FGFR1B, and FGFR1G isoforms of the receptor with the A isoform containing three Ig domains, while the B and G isoforms lack the N‑terminal IgI domain (3). Additional splicing of the IgIII domain, results in IIIa, IIIb, or IIIc isoforms (3). Only the alpha isoform has been identified for FGFR3 and FGFR4 and FGFR4 also lacks the IIIb and IIIc splicing events (4). The FGFR splice variants also exhibit distinct and varying binding affinities for different FGF ligands (2). FGFRs mediate the FGF signaling cascade which regulate developmental processes including cellular proliferation, differentiation, and migration, morphogenesis, and patterning (5). FGFRs transduce the signals through three dominant pathways including RAS/MAPK, PI3k/AKT, and PLC gamma (6). FGFR1 the most abundant FGFR and is widely expressed in many adult human tissues, but the splice variants display distinct tissue-specific differences with IIIc splice variants expressed in mesenchymal tissue (4, 7, 8). Mutations in FGFR1 or misregulation of FGFR1 mediated signaling is found in multiple diseases, with FGFR1A(IIIc) specifically upregulated, from breast and pancreatic cancer to Pfeiffer syndrome and osteoarthritis (4, 9-11). A soluble version of the FGFR1A(IIIc) splice variant has shown anti-angiogenic and anti-proliferative properties in multiple cancer cell line models (11).

Item	Value
References	Ornitz, D.M. and Itoh, N. (2015) Wiley Interdiscip. Rev. Dev. Biol. 4:215. Zhang, X. et al. (2006) J Biol. Chem. 281:15694. Ferguson, H.R. et al. (2021) Signaling Cells 10:1201. Holzmann, K. et al. (2012) J Nucleic. Acids 2012:950508. Xie, Y. et al. (2020) Sig. Transduct. Target Ther. 5:181. Mossahebi-Mohammadi, M. et al. (2020) Front Cell Dev. Biol. 18:79. Hughes, S.E. (1997) J. Histochem. Cytochem. 45:1005. Delezoide, A.L. et al. (1998) Mech. Dev. 77:19. Yamashita-Sugahara, Y. et al. (2016) Sci. Rep. 6:35908. Teven, C.M. et al. (2014) Genes Dis. 1:199. Babina, I. and Turner, N. (2017) Nat. Rev Cancer 17:318.
Long Name	Fibroblast Growth Factor Receptor 1 alpha
Alternate Names	FGF R1a, FGFR1 alpha

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Recombinant Human FGFR1 alpha (IIIc) His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human FGFR1 alpha (IIIc) His-tag Protein, CF

Scientific Data Examples for Recombinant Human FGFR1 alpha (IIIc) His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: FGFR1 alpha

Citations for Recombinant Human FGFR1 alpha (IIIc) His-tag Protein, CF

Product Documents for Recombinant Human FGFR1 alpha (IIIc) His-tag Protein, CF

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