Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF

Name: Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 658-FR
Price: 377 USD

Catalog # 658-FR | R&D Systems, Inc. a Bio-Techne Brand

Citations (5)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	NP_056934
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF

Product Specifications for Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human FGFR1 alpha protein

Human FGF R1 alpha (IIIc) (Arg22-Glu374) Accession # NP_056934	IEGRDMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Arg22

Predicted Molecular Mass

66 kDa (monomer)

SDS-PAGE

100-110 kDa, reducing conditions

Activity

Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED₅₀ for this effect is 1‑3 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

658-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR1 alpha

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. Only the alpha isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1 - 3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1 - 3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

Item	Value
References	Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669. Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.
Long Name	Fibroblast Growth Factor Receptor 1 alpha
Alternate Names	FGF R1a, FGFR1 alpha

Citations for Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay

C Yanucil et al. (2022-05-05), FGF21-FGFR4 signaling in cardiac myocytes promotes concentric cardiac hypertrophy in mouse models of diabetes Scientific Reports, 2022-012(1):7326.
PMID: 35513431
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Chan W et al. (2015-06-15), 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline. PLoS ONE, 2015-010(6):e0130147.
PMID: 26075383
Species: Bacteria
Sample Types: Trypanosoma
Applications: PcIP

Lu B et al. (2008-06-09), A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 2008-076(8):3530-8.
PMID: 18541656
Species: Human
Sample Types: Antibody
Applications: Surface Plasmon Resonance

Martinez-Torrecuadrada J et al. (2005-09-01), Targeting the extracellular domain of fibroblast growth factor receptor 3 with human single-chain Fv antibodies inhibits bladder carcinoma cell line proliferation. Clin. Cancer Res., 2005-011(17):6280-90.
PMID: 16144932
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Kessaris N et al. (2004-02-11), Cooperation between sonic hedgehog and fibroblast growth factor/MAPK signalling pathways in neocortical precursors. Development, 2004-0131(6):1289-98.
PMID: 14960493

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Showing 1 - 5 of 5 citations Showing All

Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF

Catalog # 658-FR | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: FGFR1 alpha

Citations for Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF (5)

Product Documents for Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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