What receptors does FGF basic bind?

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors

Does human FGF basic show activity on mouse cells?

Yes, it does. The bioassay uses NR-6 mouse fibroblast cells. There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Name: Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF
Brand: R&D Systems
SKU: 3718-FB
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3718-FB

Citations (6)

(1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	NP_001997
Applications:	Bioactivity

View Full Technical Details for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Complete Your Research

Product Specifications for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Source	E. coli-derived human FGF basic/FGF2/bFGF protein Ala144-Ser288
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala144
Predicted Molecular Mass	16 kDa
Activity	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 0.1-0.6 ng/mL.

Scientific Data Examples for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

	Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein Bioactivity Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W.et al. (1985) Methods Enzymol.109:749. The ED₅₀ for this effect is 0.1-0.6 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3718-FB

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: FGF basic/FGF2/bFGF

FGF basic (also known as FGF-2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF-2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4-6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).

Item	Value
References	Sorenson, V. et al. (2006) BioEssays 28:504. Kardami, E. et al. (2004) Cardiovasc. Res. 63:458. Nugent, M.A. and R.V. Lozzo (2000) Int. J. Biochem. Cell Biol. 32:115. Abraham, J.A. et al. (1986) EMBO J. 5:2523. Prats, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:1836. Arnaud, E. et al. (1999) Mol. Cell. Biol. 19:505. Foletti, A. et al. (2003) Cell. Mol. Life Sci. 60:2254. Arese, M. et al. (1999) Mol. Biol. Cell 10:1429. Pintucci, G. et al. (1996) Mol. Biol. Cell 7:1249. Nickel, W. (2005) Traffic 6:607. SwissProt # P09038. Klagsbrun, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1839. Bailly, K. et al. (2000) FASEB J. 14:333. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
Long Name	Fibroblast Growth Factor basic
Entrez Gene IDs	2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names	Basic fibroblast growth factor, FGF basic, FGF-2, FGF2, FGFBprostatropin, HBGF-2, Prostatropin, bFGF, basic fibroblast growth factor bFGF, fibroblast growth factor 2 (basic), heparin-binding growth factor 2

Citations for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF (6)

Showing 1 - 6 of 6 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF include:

Species: Human
Sample Types: Organoid
Applications: Bioassay

F Bonini et al. (2022), The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial Integration Brain sciences, 12(4).
PMID: 35447953
Species: Human
Sample Types: Protein
Applications: ELISA Capture

S Jadhav et al. (2021), A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma PLoS ONE, 16(1):e0245614.
PMID: 33481901
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay

HC Ting et al. (2021), Coactivation of GSK3beta and IGF-1 Attenuates Amyotrophic Lateral Sclerosis Nerve Fiber Cytopathies in SOD1 Mutant Patient-Derived Motor Neurons Cells, 10(10).
PMID: 34685754
Species: Human
Sample Types: Serum
Applications: Bioassay

AN Holowatyj et al. (2021), The Use of Human Serum Samples to Study Malignant Transformation: A Pilot Study Cells, 10(10).
PMID: 34685650
Species: Human
Sample Types: Cell Culture Supernates
Applications: Cell Culture

M Shibata et al. (2021), Regulation of prefrontal patterning and connectivity by retinoic acid Nature, 0(0).
PMID: 34599305
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

E Steinberg et al. (2020), Low dose amiodarone reduces tumor growth and angiogenesis Sci Rep, 10(1):18034.
PMID: 33093573

There are no citations that match your criteria.

Showing 1 - 6 of 6 citations Showing All

Customer Reviews for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 07/31/2020

Supplementing media for growing iPS-derived cortical spheroids

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

FAQs for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

What receptors does FGF basic bind?

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.bio-techne.com/resources/articles/fibroblast-growth-factors-and-their-receptors
Does human FGF basic show activity on mouse cells?

Yes, it does. The bioassay uses NR-6 mouse fibroblast cells. There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Scientific Data Examples for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: FGF basic/FGF2/bFGF

Citations for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF (6)

Customer Reviews for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF (1)

Customer Images

Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

FAQs for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Product Documents for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected