Source	Chinese Hamster Ovary cell line, CHO-derived human Fibroblast Activation Protein alpha/FAP protein Leu26-Asp760 with an N-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His
Predicted Molecular Mass	86 kDa
SDS-PAGE	83-92 kDa, under reducing conditions
Activity	Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2500 pmol/min/μg, as measured under the described conditions.

Recombinant Human FAP His-tag (Mammalian) Protein, CF Scientific Data Examples

	Recombinant Human FAP His-tag (Mammalian) Protein Enzyme Activity Recombinant Human FAP His-tag (Mammalian) (Catalog # 10921-SE) is measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC).
	Recombinant Human FAP His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human FAP His-tag Protein (Catalog # 10921-SE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-92 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10921-SE

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as seprase) is a 95 kDa Type II transmembrane serine protease that forms a homodimer and is structurally related to the dipeptidyl peptidase IV (DPPIV/CD26) family with a short cytoplasmic tail, a single transmembrane domain, and an extracellular domain that contains the active site (1-3). It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences (4,5). FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin (1,4,5) as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins) (6). FAP is also known to interact with several surface molecules to play roles in cell signaling, cell invasion and migration (3). Although not detectible in normal tissues, FAP is elevated in activated stromal fibroblasts, tumor-associated macrophages, activated hepatic stellate cells and myofibroblasts during pathological conditions that include tissue remodeling such as most types of cancer, wound healing, arthritis, atherosclerosis, and fibrosis (1,3,4,7-9). Targeting FAP with vaccines, antibodies, or pharmacologics impairs tumor progression in several cancer models making it a promising immunotherapy target (9-12).

Item	Value
References	Zi, F. et al. (2015) Mol. Med. Rep. 11:3203. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595. Lay, A.J. et al. (2019) Front Biosci. 24:1. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505. Aertgeerts, K. et al. (2005) J. Biol. Chem. 280:19441. Keane, F.M. et al. (2011) FEBS J. 278:1316. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110. Bauer, S. et al. (2006) Arthritis Res. 8:R171. Jiang, G.M. et al. (2016) Oncotarget 7:33472. Cheng, J.D. et al. (2002) Cancer Res. 62:4767. Kraman, M. et al. (2010) Science 330:827. Busek, P. et al. (2018) Front. Biosci. 23:1933.
Entrez Gene IDs	2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)
Alternate Names	170 kDa melanoma membrane-bound gelatinase, DKFZp686G13158, DPPIV, EC 3.4.21.-, FAP, FAPA, Fibroblast Activation Protein alpha, Integral membrane serine protease, Seprase, fibroblast activation protein, alpha, vibronectin

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Recombinant Human FAP His-tag (Mammalian) Protein, CF

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