Recombinant Human F-Spondin/SPON1 Protein

Name: Recombinant Human F-Spondin/SPON1 Protein
Brand: R&D Systems
SKU: 3135-SP

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3135-SP

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	Q9HCB6
Applications:	Bioactivity

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Product Specifications for Recombinant Human F-Spondin/SPON1 Protein

Source	Mouse myeloma cell line, NS0-derived human F-Spondin/SPON1 protein Phe29-Cys807, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Phe29
Predicted Molecular Mass	88.9 kDa
SDS-PAGE	110-115 kDa, reducing conditions
Activity	Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3135-SP

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3135-SP/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: F-Spondin/SPON1

F-Spondin (floor plate and thrombospondin homology), also called Spondin-1, SPON1 or VSGP (vascular smooth muscle growth-promoting factor), is an approximately 110 kDa secreted glycoprotein that is a member of a subgroup of TSR (thrombospondin) molecules that are either membrane-bound or associated with the extracellular matrix (ECM) (1-3). Human F-Spondin is synthesized as an 807 amino acid (aa) precursor with a 779 aa mature region that includes an N-terminal reelin‑like domain, an F-spondin (FS) domain, and six C‑terminal thrombospondin (TSP) type I repeats (1-3). Mature human F-Spondin shares 97%, 97%, 98% and 99% aa sequence identity with mouse, rat, bovine and canine F-Spondin, respectively. TSP 5 and 6 bind ECM, while TSP 1-4 plus the FS domain may mediate repulsive activity on motor neurons and outgrowth promoting activity on sensory neurons during development or after injury (2-5). Crystal structure indicates that the reelin-like domain binds heparin and may mediate weak dimerization (6). Plasmin cleavage generates a diffusible 95 kDa, 656 aa F-spondin that lacks TSP 5 and 6, while non‑plasmin cleavage between the FS segment and the first TSP repeat generates 60 kDa and 50 kDa fragments (3, 4, 7). F-Spondin shows unusual C‑mannosylation and O-fucosylation within the TSP repeats (3). Mammalian cells expressing F-spondin include floor plate epithelium, ventral motor neurons, Schwann cells, fibroblasts, hippocampal pyramidal cells, endothelial cells, vascular smooth muscle cells and some tumor cells (2-5, 8). F-Spondin can either tether cells to the ECM or interfere with integrin adhesion, thus either blocking or allowing nerve or vascular endothelial cell migration (3, 9). It binds beta -amyloid fibrils and inhibits beta ‑secretase cleavage, thus reducing A beta plaque deposition associated with Alzheimer’s disease (10, 11). F-Spondin is also reported to inhibit differentiation or migration during angiogenesis (affecting endothelial cells) and bone development (affecting osteoclast and chondrocyte precursors) (3, 9, 12, 13).

Item	Value
References	Miyamoto, K. et al. (2001) Arch. Biochem. Biophys. 390:93. Klar, A. et al. (1992) Cell 69:95. Feinstein, Y. and A. Klar (2004) Int. J. Biochem. Cell Biol. 36:975. Burstyn-Cohen, T. et al. (1998) J. Neurosci. 18:8875. Feinstein, Y. et al. (1999) Development 126:3637. Tan, K. et al. (2008) J. Mol. Biol. 381:1213. Tzarfaty-Majar, V. et al. (2001) J. Biol. Chem. 276:28233. Pyle-Chenault, R.A. et al. (2005) Tumor Biol. 26:245. Terai, Y. et al. (2001) J. Cell Physiol. 188:394. Ho, A. and T.C. Sudhof (2004) Proc. Natl. Acad. Sci. USA 101:2548. Hafez, D.M. et al. (2012) Neuroscience 223:465. Palmer, G.D. et al. (2010) J. Orthop. Res. 28:1323. Oka, H. et al. (2011) J. Periodontol. 82:1776.
Entrez Gene IDs	10418 (Human); 233744 (Mouse); 64456 (Rat)
Alternate Names	F-Spondin, FSpondin, KIAA0762VSGP/F-spondin, MGC10724, SPON1, VSGP, Vascular smooth muscle cell growth-promoting factor, spondin 1, (f-spondin) extracellular matrix protein, spondin 1, extracellular matrix protein, spondin-1

Citations for Recombinant Human F-Spondin/SPON1 Protein (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human F-Spondin/SPON1 Protein include:

Species: Human
Sample Types: Serum
Applications: Multiplex Standard

J Song et al. (2019), A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma Clin Proteomics, 16(0):10.
PMID: 30867659
Species: N/A
Sample Types: N/A
Applications: Western Blot

Cheng YC et al. (2009), F-spondin plays a critical role in murine neuroblastoma survival by maintaining IL-6 expression. J. Neurochem., 110(3):947-55.
PMID: 19549008

Recombinant Human F-Spondin/SPON1 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human F-Spondin/SPON1 Protein

Formulation, Preparation and Storage

Carrier Free

3135-SP

Carrier

3135-SP/CF

Carrier Free

Reconstitution Calculator

Background: F-Spondin/SPON1

Citations for Recombinant Human F-Spondin/SPON1 Protein (2)

Product Documents for Recombinant Human F-Spondin/SPON1 Protein

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Certificate of Analysis

SDS

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