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Recombinant Human Enteropeptidase/Enterokinase Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1585-SE

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1585-SE-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Enteropeptidase/Enterokinase protein
Leu41-His1019 & Ser118-His1019, with a C-terminal 9-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu41 & Ser118

Predicted Molecular Mass

110 kDa

SDS-PAGE

150 kDa, reducing conditions

Activity

Measured by its ability to cleave a colorimetric peptide substrate, Z-Lys-SBzl.
The specific activity is >10,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1585-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Enteropeptidase/Enterokinase

EK initiates activation of pancreatic proteases by converting trypsinogen to trypsin, which in turn activates chymotrypsin, carboxypeptidases and elastases. Located in intestinal brush border, it is a disulfide bond linked dimer of the heavy and light chains, which are derived from the same single-chain precursor. The multidomain‑containing the heavy chain consists of a short cytoplamic tail, a transmembrane, a SEA, a SRCR, a MAM, two CUB and two LDL-receptor class A domains. The light chain contains the catalytic domain of trypsin-like serine proteases. The purified recombinant human EK corresponds to the single-chain form starting at the end of the transmembrane domain.

Alternate Names

Enterokinase, ENTK, PRSS7, TMPRSS15

Entrez Gene IDs

5651 (Human)

Gene Symbol

TMPRSS15

UniProt

Additional Enteropeptidase/Enterokinase Products

Product Documents for Recombinant Human Enteropeptidase/Enterokinase Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Enteropeptidase/Enterokinase Protein, CF

For research use only

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