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Recombinant Human EGFR His-tag Protein, CF

Catalog # 11302-ER | R&D Systems, Inc. a Bio-Techne Brand
HEK293 Expressed
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11302-ER-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human EGFR protein
Leu25-Ser645, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

69 kDa

SDS-PAGE

94-104 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human EGFR (Research Grade Cetuximab Biosimilar) Antibody  (Catalog # MAB9577) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human EGFR His-tag (Catalog # 11302-ER) binds with an ED50 of 1.25-15.0 ng/mL.

Scientific Data Images for Recombinant Human EGFR His-tag Protein, CF

Recombinant Human EGFR His-tag Protein Binding Activity.

When Human EGFR (Research Grade Cetuximab Biosimilar) Antibody (MAB9577) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human EGFR His-tag (Catalog # 11302-ER) binds with an ED50 of 1.25-15.0 ng/mL.

Recombinant Human EGFR His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human EGFR His-tag Protein (Catalog # 11302-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 94-104 kDa.

Formulation, Preparation and Storage

11302-ER
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

Epidermal growth factor receptor (EGFR), also known as HER-1 and ErbB1, is a member of a subfamily of receptor tyrosine kinases comprised of four members: EGFR, ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD) containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing, along with a tumor specific mutant EGFRvIII, are known to exist (3-5). The ECD of mature, full-length EGFR shares 88% and 89% amino acid sequence identity with mouse and rat EGFR, respectively. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (6-8). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (6-8). EGFR is overexpressed in a wide variety of tumors, with EGFRvIII overexpressed particularly in glioblastoma multiforme (GMB) and is the target of several anti-cancer therapeutics (5,9,10).

References

  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Guillaudeau, A. et al. (2012) PLoS One. 7:1.
  4. Reiter J.L. et al. (2001) Genomics 71:1.
  5. Gan HK et al. (2013) FEBS J. 280:5350
  6. Freed, D. M. et al. (2017) Cell. 171:683.
  7. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  8. Faria, J.A. et al. (2016) BBRC. 478:39.
  9. An Z. et al. (2018) Oncogene. 37:1561.
  10. Lee, C. K. et al. (2017) J. Thoracic Oncology. 12:403.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

UniProt

Additional EGFR Products

Product Documents for Recombinant Human EGFR His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human EGFR His-tag Protein, CF

For research use only

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