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Recombinant Human E-Selectin/CD62E His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10258-ES

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10258-ES-100

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human E-Selectin/CD62E protein
Trp22-Pro556, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp22

Predicted Molecular Mass

59 kDa

SDS-PAGE

84-109 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
The ED50 for this effect is 0.2-1 μg/mL

Scientific Data Images for Recombinant Human E-Selectin/CD62E His-tag Protein, CF

Recombinant Human E-Selectin/CD62E His-tag Protein Bioactivity

Recombinant Human E-Selectin/CD62E His-tag Protein Bioactivity

Recombinant Human E-Selectin/CD62E (Catalog # 10258-ES) supports the adhesion of U937 human histiocytic lymphoma cell. The ED50 for this effect is 0.2-1 μg/mL.
Recombinant Human E-Selectin/CD62E His-tag Protein SDS-PAGE

Recombinant Human E-Selectin/CD62E His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human E-Selectin/CD62E His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-109 kDa and 60-84 kDa, respectively .

Formulation, Preparation and Storage

10258-ES
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Entrez Gene IDs

6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)

Gene Symbol

SELE

UniProt

Additional E-Selectin/CD62E Products

Product Documents for Recombinant Human E-Selectin/CD62E His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human E-Selectin/CD62E His-tag Protein, CF

For research use only

Reconstitution Calculator

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