Recombinant Human DLL4 Fc Chimera Protein, CF

Name: Recombinant Human DLL4 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10185-D4

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10185-D4

Citations (3)

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Key Product Details

Source:	HEK293
Accession #:	Q9NR61
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human DLL4 Fc Chimera Protein, CF

Product Specifications for Recombinant Human DLL4 Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human DLL4 protein

Human DLL4 (Ser27-Pro524) Accession # Q9NR61	IEGRMDP	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser27

Predicted Molecular Mass

81 kDa

SDS-PAGE

88-98 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED₅₀ fort his effect is 0.02-0.12 μg/mL.

Scientific Data Examples for Recombinant Human DLL4 Fc Chimera Protein, CF

	Recombinant Human DLL4 Fc Chimera Protein Bioactivity Recombinant Human DLL4 Fc Chimera (Catalog # 10185-D4) enhances Recombinant Human BMP-2 (Catalog # 355-BM) induced alkaline phosphatase activity in the C3H10T1/2 mouse embryonic fibroblast cell line. The ED₅₀ for this effect is 0.02-0.12 µg/mL.
	Recombinant Human DLL4 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human DLL4 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 88-98 kDa and 170-190 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10185-D4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

Item	Value
References	Shutter, J.R. et al. (2000) Genes Dev. 14:1313. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543. Walker, L. et al. (2001) Stem Cells 19:543. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
Long Name	Delta-like 4
Entrez Gene IDs	54567 (Human); 54485 (Mouse); 100152163 (Porcine)
Alternate Names	DLL4, Delta 4 precursor, Drosophila Delta homolog 4, MGC126344, delta 4, delta ligand 4, delta-like 4 (Drosophila), delta-like 4 homolog, delta-like 4 homolog (Drosophila), delta-like 4 protein, delta-like protein 4, delta4, hdelta2, notch ligand DLL4, notch ligand delta-2

Citations for Recombinant Human DLL4 Fc Chimera Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human DLL4 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Sottoriva et al. (2022), Notch-IL21 signaling axis primes bone marrow T cell progenitor expansion JCI Insight, 0(0).
PMID: 35349492
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Schmidl et al. (2022), NOTCH1 Intracellular Domain and the Tumor Microenvironment as Prognostic Markers in HNSCC Cancers, 14(4).
PMID: 35205828
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Wei et al. (2020), Notch signalling drives synovial fibroblast identity and arthritis pathology Nature, 582(7811):259-264.
PMID: 32499639

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