Recombinant Human Cystatin E/M Protein, CF
Catalog # 1286-PI | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Arg29-Met149, with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The IC50 value is approximately 7.0 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1286-PI
Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Cystatin E/M
Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily (1, 2). It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning (1, 2). However, its protein was found only in skin and sweat glands by a third group (3). In addition to being a cysteine protease inhibitor, cystatin E/M is also a substrate for transglutaminases (3). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age (4). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (5).
References
- Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
- Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
- Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.
- Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
- Hong, J. et al. (2002) J. Neurochem. 81:922.
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human Cystatin E/M Protein, CF
Product Specific Notices for Recombinant Human Cystatin E/M Protein, CF
For research use only
Citations for Recombinant Human Cystatin E/M Protein, CF (2)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Cystatin E/M Protein, CF include:
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Sample Types: Protein
Applications: Enzyme AssayJohnson GD et al. (2005-10-21), Characterization of cathepsin L secreted by Sf21 insect cells. Arch. Biochem. Biophys., 2005-1444(1):7-14.
PMID: 16274658 -
Sample Types: N/A
Applications: ELISA (Standard)Ito T et al. (2005-01-08), Murine monoclonal antibody which can distinguish cystatins SA1 and SA2. Mol. Immunol., 2005-042(10):1259-63.
PMID: 15829315
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Customer Reviews for Recombinant Human Cystatin E/M Protein, CF (1)
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