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Recombinant Human Cystatin E/M Protein, CF

Catalog # 1286-PI | R&D Systems, Inc. a Bio-Techne Brand
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1286-PI-010

Key Product Details

Accession #

Source

NS0

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Cystatin E/M protein
Arg29-Met149, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg29

Predicted Molecular Mass

15 kDa

SDS-PAGE

15 kDa and 21 kDa, reducing conditions

Activity

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009).
The IC50 value is approximately 7.0 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1286-PI
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin E/M

Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily (1, 2). It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning (1, 2). However, its protein was found only in skin and sweat glands by a third group (3). In addition to being a cysteine protease inhibitor, cystatin E/M is also a substrate for transglutaminases (3). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age (4). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (5).

References

  1. Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
  2. Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
  3. Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.
  4. Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
  5. Hong, J. et al. (2002) J. Neurochem. 81:922.

Alternate Names

CST6

Entrez Gene IDs

1474 (Human); 73720 (Mouse)

Gene Symbol

CST6

UniProt

Product Documents for Recombinant Human Cystatin E/M Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Cystatin E/M Protein, CF

For research use only

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