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Recombinant Human Complement MASP3 Catalytic Domain, CF

Catalog # 1724-SE | R&D Systems, Inc. a Bio-Techne Brand
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1724-SE-020

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Complement Factor MASP3 protein
Ile450-Val721, with an N-terminal signal peptide and a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile450

Predicted Molecular Mass

31 kDa

SDS-PAGE

43 kDa, reducing conditions

Activity

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >10,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1724-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Complement Factor MASP3

MASP3 is a member of the MASPs involved in mannan-binding lectin (MBL) complement pathway (1). The MBL pathway is initiated by the binding of MBL to specific carbohydrate structures found on the surface of a variety of microorganisms. Activation of the complement pathway via MBL is initiated by specific MASPs. Three MASPs have been identified and all have domain structures similar to those of C1r and C1s with a heavy chain (chain A) and a light chain (chain B). Chain A is composed of CUB1, EGF, CUB2, CCP1 and CCP2 while chain B corresponds to the catalytic domain found in many serine proteases. MASP1 and MASP3 are two alternatively spliced products of a single gene, which contain the same A chains but entirely different B chains. Distinct MASPs found in different MBL oligomers may have different biological activities. For example, MASP3, found together with MASP2, downregulates the C4 and C2 cleaving activity of MASP2. The protease activity of MASP3 is first revealed here using recombinant human MASP3CD (2), which is inhibited by serine protease inhibitors such as ecotin and AEBSF (Catalog # http://www.rndsystems.com/product_results.aspx?k=1328-PI">1328-PI and http://www.rndsystems.com/product_results.aspx?k=EI001">EI001).

References

  1. Dahl, M.R. et al. (2001) Immunity 15:127.
  2. Cortesio, C.L. and W. Jiang (2006) Arch. Biochem. Biophys. 449:164.

Long Name

Mannan-binding lectin-Associated Serine Protease 3

Alternate Names

MASP1

Entrez Gene IDs

5648 (Human)

Gene Symbol

MASP1

UniProt

Product Documents for Recombinant Human Complement MASP3 Catalytic Domain, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Complement MASP3 Catalytic Domain, CF

For research use only

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