Recombinant Human COMP His-tag Protein, CF

Name: Recombinant Human COMP His-tag Protein, CF
Brand: R&D Systems
SKU: 3134-CPB

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3134-CPB

Citations (4)

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Key Product Details

Source:	NS0
Accession #:	P49747
Structure / Form:	Disulfide linked homopentamer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human COMP His-tag Protein, CF

Product Specifications for Recombinant Human COMP His-tag Protein, CF

Source	Mouse myeloma cell line, NS0-derived human COMP/Thrombospondin-5 protein Gln21-Ala757 (Ala256Arg), with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gln21
Predicted Molecular Mass	81.8 kDa
SDS-PAGE	106-118 kDa, reducing conditions
Activity	Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Recombinant Human COMP/Thrombospondin‑5 immobilized at 10 µg/mL (100 µL/well) will induce more than 40% of ATDC-5 cell adhesion.

Scientific Data Examples for Recombinant Human COMP His-tag Protein, CF

	Recombinant Human COMP His-tag Protein Bioactivity Immobilized Recombinant Human COMP/Thrombospondin‑5 (Catalog # 3134-CPB) at 10 µg/mL (100 µL/well) induces more than 40% cell adhesion.
	Recombinant Human COMP His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Human COMP/Thrombospondin‑5 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-118 kDa and 500 - 550 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3134-CPB

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: COMP/Thrombospondin-5

Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). The human COMP cDNA encodes a 757 amino acid (aa) precursor that includes a 20 aa signal sequence followed by a non-collagenous coiled‑coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain (5). Human COMP shares 86-93% aa sequence identity with rat, mouse, equine, bovine, and canine COMP. Within the TSP type-3 repeats and TSP C-terminal domain, human COMP shares 60%, 61%, 74%, and 80% aa sequence identity with human Thrombospondin-1, -2, -3, and -4, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D₃ which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type‑3 repeat, results in diminished calcium binding ability (13, 14).

Item	Value
References	Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961. Posey, K.L. et al. (2004) Int. J. Biochem. Cell Biol. 36:1005. Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294. Newton, G. et al. (1994) Genomics, 24:435. DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422. Efimov, V.P. et al. (1994) FEBS Lett. 341:54. Ozbek, S. et al. (2002) EMBO J. 21:5960. Chen, F.H. et al. (2005) J. Biol. Chem. 280:32655. Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278. Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296. Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207. Kennedy, J. et al. (2005) Eur. J. Hum. Genet. 13:547. Hou, J. et al. (2000) Cell Calcium 27:309.
Long Name	Cartilage Oligomeric Matrix Protein
Entrez Gene IDs	1311 (Human); 12845 (Mouse)
Alternate Names	COMP, EDM1, EPD1, MED, MEDMGC131819, MGC149768, PSACH, THBS5, TSP5, Thrombospondin-5, Thrombospondin5, cartilage oligomeric matrix protein, cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple), cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple), pseudoachondroplasia (epiphyseal dysplasia 1, multiple)

Citations for Recombinant Human COMP His-tag Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human COMP His-tag Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Sun et al. (2019), Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP J. Exp. Clin. Cancer Res., 38(1):170.
PMID: 30999932
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Q Li et al. (2018), HSCs-derived COMP drives hepatocellular carcinoma progression by activating MEK/ERK and PI3K/AKT signaling pathways J. Exp. Clin. Cancer Res., 37(1):231.
PMID: 30231922
Sample Types: Complex Sample Type
Applications: Bioassay

A Raj et al. (2017), Molecular synergy in biolubrication: The role of cartilage oligomeric matrix protein (COMP) in surface-structuring of lubricin J Colloid Interface Sci, 495(0):200-206.
PMID: 28208081
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Jeschke A et al. (2015), Deficiency of Thrombospondin-4 in Mice Does Not Affect Skeletal Growth or Bone Mass Acquisition, but Causes a Transient Reduction of Articular Cartilage Thickness. PLoS ONE, 10(12):e0144272.
PMID: 26629997

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