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Recombinant Human CLEC2L Fc Chimera Protein, CF

Catalog # 10204-CL | R&D Systems, Inc. a Bio-Techne Brand
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10204-CL-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CLEC2L protein
MD Human IgG2A
(Pro100-Lys333)
IEGR Human CLEC2L
(Ser90-Thr214)
Accession # P0C7M8-1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

41 kDa

SDS-PAGE

39-44 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Human Galectin-3 (Catalog # 1154-GA) that produces 50% of the optimal binding response is 1.5-12 μg/mL.

Scientific Data Images for Recombinant Human CLEC2L Fc Chimera Protein, CF

Recombinant Human CLEC2L Fc Chimera Protein Binding Activity

Recombinant Human CLEC2L Fc Chimera Protein Binding Activity

When Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) is immobilized at 5 μg/mL, 100 μL/well, Recombinant Human Galectin-3 (Catalog # 1154-GA) binds with an ED50 of 1.5-12 μg/mL.
Recombinant Human CLEC2L Fc Chimera Protein SDS-PAGE

Recombinant Human CLEC2L Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CLEC2L Fc Chimera (Catalog # 10204-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-44 kDa and 80-90 kDa, respectively.

Formulation, Preparation and Storage

10204-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC2L

C-type lectin domain family 2, member L (CLEC2L), is a type II transmembrane protein of the CLEC2 family of proteins that includes CD69, LLT1, AICL and KACL (1, 2). CLEC2 family members have broad tissue expression, with LLT1, AICL and KACL expressed on B cells, monocytes, and keratinocytes, respectively (3-5). In contrast, CLEC2L, a homodimeric cell surface receptor, is predominantly expressed in the brain and is referred to as the brain-associated C-type lectin (BACL) (6). Mature human CLEC2L includes a 68 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 125 aa extracellular domain (ECD). Within the ECD, human CLEC2L shares 94% aa sequence identity with mouse and rat CLEC2L. Our assay shows that CLEC2L binds to Galectin-3.

References

  1. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
  2. Vogler, I. and A. Steinle (2011) J. Innate Immune. 3:227.
  3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
  4. Spreu, J. et al. (2010) Proc. Natl. Acad. Sci. USA 107:5100.
  5. Rosen, D.B. et al. (2008) J. Immunol. 180:6508.
  6. Lysenko, O. et al. (2013) PloS One. 8:e65345.

Long Name

C-type Lectin Domain Family 2, Member L

Alternate Names

C-type lectin domain family 2 member L, C-type lectin domain family 2, member L

Entrez Gene IDs

154790 (Human); 665180 (Mouse); 296985 (Rat)

Gene Symbol

CLEC2L

UniProt

Product Documents for Recombinant Human CLEC2L Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CLEC2L Fc Chimera Protein, CF

For research use only

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