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Recombinant Human CLEC17A Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10147-CL

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10147-CL-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CLEC17A protein
MD Human IgG1
(Pro100-Lys330)
IEGR Mouse CLEC17A
(Lys194-Cys378)
Accession # Q6ZS10-1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

48 kDa

SDS-PAGE

54-66 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells.
The ED50 for this effect is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Human CLEC17A Fc Chimera Protein, CF

Recombinant Human CLEC17A Fc Chimera Protein Bioactivity

Recombinant Human CLEC17A Fc Chimera Protein Bioactivity

Immobilized Recombinant Human CLEC17A Fc Chimera (Catalog # 10147-CL) supports the adhesion of the MCF‑7 human breast cancer cells. The ED50 for this effect is 1.5-9 μg/mL.
Recombinant Human CLEC17A Fc Chimera Protein SDS-PAGE

Recombinant Human CLEC17A Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CLEC17A Fc Chimera (Catalog # 10147-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-66 kDa and 110-130 kDa, respectively.

Formulation, Preparation and Storage

10147-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC17A

C-type lectin domain family 17, member A (CLEC17A), also known as Prolectin, is type II transmembrane protein that is expressed mainly on dividing B cells found in the germinal centers of secondary lymphoid organs, including lymph nodes, tonsils, stomach, intestine, appendix and spleen (1, 2). CLEC17A binds preferentially to epithelial rather than to mesenchymal cells, and it behaves as a cell adhesion molecule for epithelial cells (2).  It has high specificity towards mannose and was found to form disulfide-linked oligomers (1, 3). Human CLEC17A is synthesized as a 378 amino acid (aa) protein that includes a 172 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 185 aa extracellular domain (ECD). Within the ECD, human CLEC17A shares 84% aa sequence identity with canine CLEC17A. C-type lectins are Ca2+-depending sugar-binding proteins that are involved in several immune-related and other physiological functions. Presently, 17 groups within the C-type lectin superfamily have been recognized (4), with more C-type lectins being constantly discovered based on the presence of a conserved 115-130 amino acid domain along their sequences - the C-type carbohydrate recognition domain (CRD). However, for most of the recently identified C-type lectins, their interactions with carbohydrates, intracellular functions and molecular mechanisms still remain unclear (3).

References

  1. Graham, S. A. et al. (2009) J. Biol. Chem. 284:18537.
  2. Breiman, A. et al. (2016) Oncotarget 7:14064.
  3. Koh, G. et al. (2011) BMC Bioinformatics 12:S5.
  4. Zelensky AN. et al. (2005) FEBS Journal 272:6179.

Long Name

C-type Lectin Domain Family 17, Member A

Alternate Names

Prolectin

Entrez Gene IDs

388512 (Human)

Gene Symbol

CLEC17A

UniProt

Additional CLEC17A Products

Product Documents for Recombinant Human CLEC17A Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CLEC17A Fc Chimera Protein, CF

For research use only

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