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Recombinant Human CLEC-12B Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1652-CL

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1652-CL-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CLEC12B protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human CLEC12B
(Leu65-Asp276)
Accession # Q2HXU8-1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

51 kDa

SDS-PAGE

54-66 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CLEC12B Fc Chimera is immobilized at 4 µg/mL (100 µL/well), Recombinant Human MICA Fc Chimera (Catalog # 1300-MA) binds with an ED50 of 1-6 μg/mL.

Scientific Data Images for Recombinant Human CLEC-12B Fc Chimera Protein, CF

Recombinant Human CLEC-12B Fc Chimera Protein Binding Activity

Recombinant Human CLEC-12B Fc Chimera Protein Binding Activity

When Recombinant Human CLEC12B Fc Chimera (Catalog # 1652-CL) is coated onto a microplate at 4 µg/mL, Recombinant Human MICA Fc Chimera (Catalog # 1300-MA) binds with an ED50 of 1-6 µg/mL.
Recombinant Human CLEC-12B Fc Chimera Protein SDS-PAGE

Recombinant Human CLEC-12B Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CLEC12B was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-66 kDa and 110-135 kDa, respectively.

Formulation, Preparation and Storage

1652-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CLEC12B

C-type lectin domain family 12 member B (CLEC12B) is a member of the C-type lectin-like family of proteins. CLEC12B is widely expressed at low levels in various human tissues except in the brain (1, 2). A truncated version lacking a portion of the carbohydrate-recognition domain (CRD) has been detected in mammary gland, lung and ovary, and was predicted to be nonfunctional (1). CLEC12B is a cell surface receptor that may play a role in viral recognition and modulate signaling cascades due to the presence of an ITIM motif within its cytoplasmic tail (1-3). Human CLEC12B is synthesized as a 276 amino acid (aa) protein that includes a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 212 aa extracellular domain (ECD). Within the ECD, human CLEC12B shares 74% and 70% aa sequence identity with mouse and rat CLEC12B, respectively. The extracellular domain of CLEC12B shows considerable homology to the activating natural killer cell receptor NKG2D, and it antagonizes NKG2D mediated signaling through the ITIM motif (1). CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. They play a role in HIV-1, mycobacterial, and Candida infections, and the coevolution of hosts and pathogens (4). Pathogen recognition by C-type lectins triggers signaling pathways that lead to the expression of specific cytokines which subsequently instruct adaptive T helper immune responses (4).

References

  1. Hoffmann, S. et al. (2007) J. Biol. Chem. 282:22370.
  2. Huysamen, C. et al. (2009) FEMS Microbiol. Lett. 290:121.
  3. Monteiro, J.T. and B. Lepenies (2017) Viruses 9:59.
  4. van den Berg, L.M. et al. (2012) Ann N Y Acad Sci. 1253:149.

Long Name

C-type Lectin Domain Family 12, Member B

Alternate Names

Macrophage Antigen H, UNQ5782

Entrez Gene IDs

387837 (Human); 71183 (Mouse); 502900 (Rat)

Gene Symbol

CLEC12B

UniProt

Additional CLEC12B Products

Product Documents for Recombinant Human CLEC-12B Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CLEC-12B Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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