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Recombinant Human CLC/CNTFR alpha Chimera Protein

Bio-Techne includes R&D Systems | Catalog # 2415-CR

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Carrier Free
2415-CR-025/CF

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With Carrier
2415-CR-025

Key Product Details

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CLC/CNTF R alpha Chimera protein
Human
CNTF R alpha
(Gln23 - Pro346)
Accession # P26992
GSGSSRGGSGSGGSGGGGSKL Human CLC
(Leu28 - Phe225)
Accession # Q9UBD9
6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln23 predicted

Predicted Molecular Mass

61 kDa

SDS-PAGE

85-93 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 10-25 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2415-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2415-CR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLC/CNTF R alpha Chimera

Cardiotrophin-like cytokine (CLC), (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3) is a 22 - 25 kDa member of the IL-6 family of cytokines (1 - 3). As such, it is expressed as a long, type I cytokine with four alpha-helices in its structure (2). Human CLC is synthesized as a 225 amino acid (aa) precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for Ciliary Neurotrophic Factor Receptor alpha (CNTF R alpha) and Cytokine Receptor-like Factor (CLF) (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently complexed to either CLF or soluble CNTF R alpha (5, 6). Once complexed, CLC signals through a tripartite receptor complex composed of gp130, LIF R beta and CNTF R alpha (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8). Human to mouse, mature CLC shares 96% aa sequence identity. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9 - 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).

References

  1. Murakami, M. et al. (2004) Growth Factors 22:75.
  2. Ernst, M. and B.J. Jenkins (2004) Trends Genet. 20:23.
  3. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
  4. Perret, D. et al. (2003) J. Biol. Chem. 279:43961.
  5. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
  6. Elson, G.C.A. et al. (2000) Nat. Neurosci. 3:867.
  7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
  8. Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.
  9. Forger, N.G. et al. (2003) J. Neurosci. 23:8854
  10. Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.
  11. Senaldi, G. et al. (2002) J. Immunol. 168:5690.
  12. de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.

Alternate Names

CNTFR

Additional CLC/CNTF R alpha Chimera Products

Product Documents for Recombinant Human CLC/CNTFR alpha Chimera Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CLC/CNTFR alpha Chimera Protein

For research use only

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