Product Specifications for Recombinant Human Chromogranin A His-tag Protein, CF
Human embryonic kidney cell, HEK293-derived human Chromogranin A protein Leu19-Gly457, with a C-terminal 6-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
60-80 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. Recombinant Human SCG3 His-tag (Catalog # 10374-SC) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Chromogranin A His‑tag (Catalog # 10422-CH) binds with an ED50 of 40‑360 ng/mL.
Scientific Data Examples for Recombinant Human Chromogranin A His-tag Protein, CF
Recombinant Human Chromogranin A His-tag Protein Binding Activity
When Recombinant Human SCG3 His-tag (10374-SC) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Chromogranin A His-tag (10422-CH) binds with an ED50 of 40-360 ng/mL.
Recombinant Human Chromogranin A His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human Chromogranin A His-tag Protein (10422-CH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 200 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chromogranin A
Chromogranin A (CgA), also known as pituitary secretory protein I (SP-I), is a member of the granin family of regulated secretory proteins. CgA shares several protein characteristics common to the granin family: acidic isoelectric point, the capacity to bind calcium ions, the ability to form aggregates and multiple dibasic cleavage sites. Mature human CgA is 439 amino acids (aa) and contains 10 dibasic, proteolytic cleavage sites, capable of yielding several smaller peptides, each displaying a unique function (4). Mature human CgA shares 63% aa sequence identity with mouse and rat CgA. CgA is expressed exclusively in the secretory dense core granules of most normal and neoplastic neuroendocrine cells (1-3). Increased levels of CgA have been detected inpatients with neuroendocrine tumors as well as non-neuroendocrine tumors, hence CgA is an important serological marker for tumor diagnosis and monitoring tumorprogression/regression (5, 6). It has been demonstrated in mouse model that full-length CgA containing its C-terminal region can impair angiogenesis and tumor growth (5). In addition, CgA can bind to Secretogranin III to regulate the biogenesis of secretory granules (7).
Bajetta, E. et al. (1999) Cancer 86:858.
Taupenot, L. et al. (2003) N. Engl. J. Med. 348:1134.
Modlin, I.M. et al. (2010) Am. Surg. Oncol. 17:2427.
Marotta, V. et al. (2018) Endocr Relat Cancer 25:R11
Curnis, F. et al. (2016) Oncotarget 7:72716.
Gkolfinopoulos, S. et al. (2017) World J. Methodol. 7:9.
Hosaka, M. et al. (2004) J Biol Chem 279:3627.
Entrez Gene IDs
1113 (Human); 12652 (Mouse); 24258 (Rat)
Betagranin, CGA, CHGA, Catestatin, Chromofungin, Chromogranin A, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, betagranin (N-terminal fragment of chromogranin A), chromogranin A (parathyroid secretory protein 1), chromogranin-A
Citations for Recombinant Human Chromogranin A His-tag Protein, CF
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Product Documents for Recombinant Human Chromogranin A His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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