Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF

Catalog # 10301-SR | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

P34810

Source

NS0

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD68/SR-D1 protein
Human CD68/SR-D1
(Asn22-Ile320)
Accession # P34810		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn22

Predicted Molecular Mass

58 kDa

SDS-PAGE

116-130 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human S100A8 (Catalog # 9876-S8) is immobilized at 10 µg/mL (100 µL/well), it binds to Recombinant Human CD68/SR‑D1 Fc Chimera with an ED50 of 5-30 μg/mL.

Scientific Data Images for Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF

Recombinant Human CD68/SR-D1 Fc Chimera Protein SDS-PAGE

Recombinant Human CD68/SR-D1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD68/SR-D1 Fc Chimera (Catalog # 10301-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 116-130 kDa and 230-260 kDa, respectively.

Formulation, Preparation and Storage

10301-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD68/SR-D1

CD68, also called Scavenger Receptor D1 (SR-D1) and LAMP-4, is a heavily glycosylated type I transmembrane glycoprotein that belongs to the LAMP family (1). Human CD68 contains 354 amino acids (aa), with a 21 aa signal sequence and a 298 aa extracellular domain (ECD), a 25 aa transmembrane domain, and a 10 aa cytoplasmic domain. The ECD of CD68 has 9 glycosylation sites and a mucin-like region in aa 23-140. Within the ECD, human CD68 shares a 65% and 67% amino acid sequence identity with that of mouse and rat CD68, respectively. CD68 is expressed on monocytes and tissue macrophages and serves as a scavenger receptor for oxidized LDL (2). In contrast to other members of the LAMP-family of glycoproteins which are ubiquitously expressed in all cell types, CD68 appeared to have a more restricted, cell-type specific pattern of expression (3 ,4). CD68 is also a biomarker of rheumatoid arthritis and Hodgkin's lymphoma (5, 6). CD68 influences the functions of cells through NF-kB/focal adhesion kinase pathway (7). In addition, CD68 on macrophages binds tightly to both S100A8 and S100A9 to enhance the cell immunity (8).

References

  1. Holness, C.L., Simmons, D.L. (1993) Blood 81:1607.
  2. Ramprasad, M.P. et al. (1996) Proc. Natl. Acad. Sci. U. S. A. 93:14838.
  3. Gottfried, E. et al. (2008) Scand. J. Immunol. 67:453
  4. Chistiakov, D. et al. (2016) Lab Invest. 97:13.
  5. Bresnihan, B. et al. (2009) J Rheumatol. 36:1800.
  6. Touati, M. et al. (2015) 56:332.
  7. Wang, H. et al. (2014) Cancer Lett. 345:91.
  8. Okada, K. et al. (2016) J. of Leukoc. Bio. 100:1093.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Entrez Gene IDs

968 (Human); 12514 (Mouse); 287435 (Rat)

Gene Symbol

CD68

UniProt

P34810

Product Documents for Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF

For research use only

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