Recombinant Human CD52 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9116

Biotinylated
R&D Systems, part of Bio-Techne
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AVI9116-050
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Key Product Details

Source

CHO

Accession #

P31358.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD52 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD52 protein
Human CD52
(Gly25-Ser36)
Accession # P31358.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser24 & Gly25

Predicted Molecular Mass

30 kDa

SDS-PAGE

38-44 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human SIGLEC-10 Fc Chimera Protein  (Catalog # 2130-SL) is immobilized at 5.0 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CD52 Fc Chimera Avi-tag (Catalog # AVI9116) that produces 50% of the optimal binding response is 0.500-5.00 µg/mL.

Scientific Data Images for Recombinant Human CD52 Fc Chimera Avi-tag Protein, CF

Recombinant Human CD52 Fc Chimera Avi-tag Protein Bioactivity.

When Recombinant Human SIGLEC-10 Fc Chimera Protein (2130-SL) is immobilized at 5.0 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CD52 Fc Chimera Avi-tag (Catalog # AVI9116) that produces 50% of the optimal binding response is 0.500-5.00 µg/mL.

Recombinant Human CD52 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD52 Fc Chimera Avi-tag Protein (Catalog # AVI9116) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-44 kDa and 80-90 kDa, respectively.

Formulation, Preparation and Storage

AVI9116
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD52

CD52, also known as CAMPATH-1 antigen, HE5, and gp20, is a cell surface glycoprotein that can be targeted to induce immune suppression by complement-mediated cell lysis (1, 2). Mature human CD52 is a 12 amino acid peptide that is tethered to the cell surface with a GPI linkage (3). It carries a large tissue-specific carbohydrate structure which increases the molecular weight of CD52 to 15-20 kDa (3-5). CD52 is expressed on lymphocytes, monocytes, monocycte-derived dendritic cells, eosinophils, and neutrophils (6-8), as well as on mature spermatozoa and epithelial cells lining the male genital tract (5, 9, 10). It protects sperm against anti-sperm antibodies by binding to C1q and inhibiting complement activation (11). CD52 ligation on CD4+ T cells induces a suppressive population of cells that release soluble CD52 which then binds to Siglec-10 (12-14). This interaction inhibits the proliferation of activated T cells and the cytotoxic function of autoimmune CD8+ T cells in type 1 diabetes (13). Our Avi-tag Biotinylated human CD52 Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Morris, P.J. and N.K. Russell (2006) Transplantation 81:1361.
  2. Redpath, S. et al. (1998) Immunology 93:595.
  3. Xia, M.Q. et al. (1991) Eur. J. Immunol. 21:1677.
  4. Treumann, A. et al. (1995) J. Biol. Chem. 270:6088.
  5. Schroter, S. et al. (1999) J. Biol. Chem. 274:29862.
  6. Ratzinger, G. et al. (2003) Blood 101:1422.
  7. Elsner, J. et al. (1996) Blood 88:4684.
  8. Ambrose, L.R. et al. (2009) Blood 114:3052.
  9. Focarelli, R et al. (1998) Mol. Hum. Reprod. 4:119.
  10. Hale, G. et al. (1993) J. Reprod. Immunol. 23:189.
  11. Hardiyanto, L. et al. (2012) J. Reprod. Immunol. 94:142.
  12. Watanabe, T. et al. (2006) Clin. Immunol. 120:247.
  13. Bandala-Sanchez, E. et al. (2013) Nat. Immunol. 14:741.
  14. Toh, B.-H. et al. (2013) Cell. Mol. Immunol. 10:379.

Alternate Names

CAMPATH-1, CD52, CDw52, He5, HEL-S-171mP

Entrez Gene IDs

1043 (Human); 23833 (Mouse)

Gene Symbol

CD52

UniProt

P31358.1

Additional CD52 Products

Product Documents for Recombinant Human CD52 Fc Chimera Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD52 Fc Chimera Avi-tag Protein, CF

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