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Recombinant Human CD44v5 Fc Chimera Protein, CF

Catalog # 10146-CD | R&D Systems, Inc. a Bio-Techne Brand
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10146-CD-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD44 protein
Human CD44v5
(Gln21-Thr222)
NVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHST
(Arg223-Trp269)
Accession # P16070-12
IEGRMD Human IgG1
(Pro100-Lys330)

N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln21 inferred from enzymatic pyroglutamate treatment revealing Il22.

Predicted Molecular Mass

58 kDa

SDS-PAGE

95-110 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD44v5 Fc Chimera (Catalog # 10146-CD) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Hyaluronan binds with an ED50 of 5‑30 ng/mL.

Scientific Data Images for Recombinant Human CD44v5 Fc Chimera Protein, CF

Recombinant Human CD44v5 Fc Chimera Protein Binding Activity

Recombinant Human CD44v5 Fc Chimera Protein Binding Activity

When Recombinant Human CD44v5 Fc Chimera (Catalog # 10146-CD) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Hyaluronan binds with an ED50 of 5‑30 ng/mL.
Recombinant Human CD44v5 Fc Chimera Protein SDS-PAGE

Recombinant Human CD44v5 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD44v5 Fc Chimera (Catalog # 10146-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-110 kDa and 190-220 kDa, respectively.

Formulation, Preparation and Storage

10146-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1‑3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan‑binding disulfide‑stabilized link region and a 325‑530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 transcripts undergo complex alternative splicing, and, within the stem, ten variably spliced exons (v1‑10 corresponding to exons 6‑15; although human CD44 lacks v1/exon 6) produce multiple protein isoforms (1‑4). The standard or hematopoietic form, CD44H, does not include the variable segments (1‑4). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 4, 5). Human CD44v5 contains exon 10 and is associated with tumor progression and metastasis in many types of cancer including breast, colon, lung, renal, skin, and ovarian tumors (6, 12). With variable N‑ and O‑glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21‑222), human CD44 shares 76%, 76%, 86%, 83% and 79% identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a "platform" for growth factors and metalloproteinases. Second, CD44 can function as a co‑receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (6) or may be cleaved at multiple sites by either membrane‑type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (8, 9). The cellular portion may then undergo gamma secretase‑dependent intramembrane cleavage to form an A beta‑like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (10, 11). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 8). CD44v5 plays important role in cancer progression involving in cell migration and invasion and is considered a functional cancer biomarker (6, 12).

References

  1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
  2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
  3. Screaton, G.R. et al. (1993) J. Biol. Chem. 268:12235.
  4. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
  5. Todaro, M. et al. (2014) Cell stem cell 14:342
  6. Vizoso, F.J. et al. (2004) J. Cancer Res. Clin. Oncol. 130:679.
  7. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
  8. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
  9. Nakamura, H. et al. (2004) Cancer Res. 64:876.
  10. Murakami, D. et al. (2003) Oncogene 22:1511.
  11. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
  12. Fox, S.B. et al. (1994) Cancer Res. 54:4539.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Gene Symbol

CD44

UniProt

Product Documents for Recombinant Human CD44v5 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD44v5 Fc Chimera Protein, CF

For research use only

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