Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1557-CD

Catalog #
Availability
Size / Price
Qty
Loading...
1557-CD-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD320/TCblR/8D6A protein
Human CD320
(Leu31-Val231)
Accession # Q9NPF0
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu31 and Ser36

Predicted Molecular Mass

47.2 kDa (monomer)

SDS-PAGE

80-90 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD320/TCblR/8D6A Fc Chimera is immobilized at 25 ng/mL (100 μL/well), the concentration of Recombinant Human Transcobalamin II (Catalog # 7895-TC) that produces 50% of the optimal binding response is 0.5-3 ng/mL.

Scientific Data Images for Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF

Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein Bioactivity

Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein Bioactivity

When Recombinant Human CD320/TCblR/8D6A Fc Chimera (Catalog # 1557-CD) is coated at 25 ng/mL, Recombinant Human Transcobalamin II (Catalog # 7895-TC) binds with an ED50 of 0.5-3 ng/mL.

Formulation, Preparation and Storage

1557-CD
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD320/TCblR/8D6A

CD320, also known as the Transcobalamin II receptor (TCb1R) and 8D6A, is an approximately 60 kDa transmembrane member of the LDL receptor family (1). Mature human CD320 consists of a 199 amino acid (aa) extracellular domain with two cysteine-rich LDLR-A repeats, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2). Within the extracellular domain, human CD320 shares 52% aa sequence identity with mouse and rat CD320. CD320 is a heavily glycosylated protein that is expressed as a noncovalent dimer in the kidney, placenta, liver, intestine, and secondary lymphoid organs (3 - 5). It functions as an endocytic receptor for circulating complexes of Cobalamin (Vitamin B12) and Transcobalamin II (3). In the immune system, CD320 is expressed by germinal center B cells and follicular dendritic cells (FDC) and provides a co‑stimulatory signal to B cells during the germinal center reaction (2, 6 - 8). CD320 also cooperates with CD44 in promoting the development of B cell lymphomas (9).

References

  1. May, P. et al. (2007) Ann. Med. 39:219.
  2. Li, L. et al. (2000) J. Exp. Med. 191:1077.
  3. Quadros, E.V. et al. (2009) Blood 113:186.
  4. Bose, S. et al. (1995) J. Biol. Chem. 270:8152.
  5. Park, H.J. et al. (2009) Immune Netw. 9:138.
  6. Cho, W. et al. (2008) BMC Rep. 41:863.
  7. Zhang, X. et al. (2001) J. Immunol. 167:49.
  8. Park, C.-S. and Y.S. Choi (2005) Immunology 114:2.
  9. Li, L. et al. (2004) Blood 104:815.

Long Name

Transcobalamin II Receptor

Alternate Names

8D6A, CD320, TCblR

Entrez Gene IDs

51293 (Human); 54219 (Mouse); 362851 (Rat)

Gene Symbol

CD320

UniProt

Additional CD320/TCblR/8D6A Products

Product Documents for Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=