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Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF

Catalog # 10479-CD | R&D Systems, Inc. a Bio-Techne Brand
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10479-CD-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD300e/LMIR6 protein
Human CD300e/LMIR6
(Leu18-Leu169)
Accession # Q496F6.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu18

Predicted Molecular Mass

44 kDa

SDS-PAGE

45-58 kDa and 106-125 kDa (non-reducible dimer), under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect 0.6-6.0 μg/mL.

Scientific Data Images for Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF

Recombinant Human CD300e/LMIR6 Fc Chimera Protein Bioactivity

Recombinant Human CD300e/LMIR6 Fc Chimera Protein Bioactivity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect is 0.6-6.0 μg/mL.
Recombinant Human CD300e/LMIR6 Fc Chimera Protein SDS-PAGE

Recombinant Human CD300e/LMIR6 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD300e/LMIR6 Fc Chimera Protein (10479-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-58 kDa/106-125 kDa (non-reducible dimer) and 106-125 kDa, respectively.

Formulation, Preparation and Storage

10479-CD
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300e/LMIR6

CD300e, also known as LMIR6, IREM-2, and CMRF35-like 2 (CLM-2), is an approximately 35-kDa type I transmembrane glycoprotein in the LMIR family of immune regulatory proteins (1). It consists of a 156 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and an 11 aa cytoplasmic stub (2). The ECD contains an Ig-like V-type domain with a single disulfide bond and an N-linked glycosylation site. Within the ECD, human CD300e shares 57% sequence identity with mouse and rat CD300e. Its transmembrane segment contains a charged lysine residue which mediates CD300e association with the signaling adaptor molecule DAP12 and enables CD300e to function as an activating receptor (2, 3, 5). The ligand of CD300e is unknown; sphingomyelin has been demonstrated to bind human and mouse CD300e (5). CD300e is highly expressed on intermediate and non-classical monocytes, and myeloid dendritic cells (3, 4). CD300e on monocytes from HIV-infected patients under cART is correlated with markers of disease progression and immune inflammation (6). The cross-linking of CD300e enhances LPS-mediated cytokine production by monocytes, enhancing TNF-alpha production while no enhancement is observed on IL-1 beta, IL-6 and IL-10 (7). Our in-house data indicate that CD300e inhibits T cell activation, including anti-CD3-induced IL-2 and IFN-gamma secretion.

References

  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Chung, D.H. et al. (2003) J. Immunol. 141:6541.
  3. Aguilar, H. et al. (2004) J. Immunol. 173:6703.
  4. Brckalo, T. et al. (2010) Eur. J. Immunol. 40:722.
  5. Isobe, M. et al. (2018) J. Biol. Chem. 293:3793.
  6. Vitalle, J. et al. (2017) Front. Immunol. 8:836.
  7. Zenarruzabeitia O. et al. (2016) Sci Rep. 6:32693.

Alternate Names

CD300e, CD300LE, CLM2, IREM-2, LMIR6

Entrez Gene IDs

342510 (Human); 217306 (Mouse)

Gene Symbol

CD300E

UniProt

Product Documents for Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD300e/LMIR6 Fc Chimera Protein, CF

For research use only

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