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Recombinant Human CD300b/LMIR5 Fc Chimera Protein, CF

Catalog # 1522-LM | R&D Systems, Inc. a Bio-Techne Brand
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1522-LM-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD300b/LMIR5 protein
Human CD300b
(Ile55-His187)
Accession # AAH28091
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile55

Predicted Molecular Mass

42 kDa

SDS-PAGE

46-59 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD300b/LMIR5 Fc Chimera is immobilized at 2 µg/mL, 100 µL/well, Recombinant Human LMIR2/CD300C Fc Chimera (Catalog # 3256-LM) binds with an ED50 of 1.5-9 μg/mL.

Scientific Data Images for Recombinant Human CD300b/LMIR5 Fc Chimera Protein, CF

Recombinant Human CD300b/LMIR5 Fc Chimera Protein Binding Activity

Recombinant Human CD300b/LMIR5 Fc Chimera Protein Binding Activity

When Recombinant Human CD300b/LMIR5 Fc Chimera (Catalog # 1522-LM) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LMIR2/CD300C Fc Chimera (Catalog # 3256-LM) binds with an ED50 of 1.5-9 µg/mL.
Recombinant Human CD300b/LMIR5 Fc Chimera Protein SDS-PAGE

Recombinant Human CD300b/LMIR5 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD300b/LMIR5 Fc Chimera (Catalog # 1522-LM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 46-59 kDa and 95 -120 kDa, respectively.

Formulation, Preparation and Storage

1522-LM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300b/LMIR5

LMIR5, also known as CD300b, CD300LB, CLM-7, and IREM‑3, is a glycoprotein member of the immunoglobulin superfamily (1). Human LMIR5 consists of a 134 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 amino acid (aa) transmembrane segment, and a 29 aa cytoplasmic domain (2). Within the ECD, human LMIR5 shares 51 % sequence identity with mouse and 57% aa sequence identity with rat LMIR5. The transmembrane segment contains a positively charged lysine which enables the association of LMIR5 with DAP12, DAP10, and potentially other adaptor proteins (2, 3). The cytoplasmic domain of human LMIR5 contains a phosphorylatable tyrosine motif, while that of mouse LMIR5 does not (2). LMIR5 is expressed on the surface of myeloid lineage cells (2-4). It forms noncovalent cis‑homodimers and cis-heterodimers with other CD300 family proteins, and the composition of these dimers affects the cellular response (2, 5). Antibody cross‑linking of LMIR5 induces mast cell granule release and cytokine production as well as its tyrosine phosphorylation of LMIR5 (in human) (2, 3). LMIR5 interacts with TIM1 and TIM4 which regulate T cell activation and are themselves binding partners (6). TIM1 interactions with LMIR5 mediate mast cell activation and the accumulation of neutrophils at sites of TIM1 up‑regulation on damaged renal tubule epithelial cells (6).

References

  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Martinez-Barriocanal, A. et al. (2006) J. Immunol. 177:2819.
  3. Yamanishi, Y. et al. (2008) Blood 111:688.
  4. Wu, Y. et al. (2011) Cell. Immunol. 268:68.
  5. Martinez-Barriocanal, A. et al. (2010) J. Biol. Chem. 285:41781.
  6. Yamanishi, Y. et al. (2010) J. Exp. Med. 207:1501.

Long Name

Leukocyte Mono Ig-like Receptor 5

Alternate Names

CD300b, CD300LB, CLM7, Gm253, LMIR5, TREM-5

Entrez Gene IDs

124599 (Human); 217304 (Mouse)

Gene Symbol

CD300LB

UniProt

Product Documents for Recombinant Human CD300b/LMIR5 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD300b/LMIR5 Fc Chimera Protein, CF

For research use only

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