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Recombinant Human CD25/IL-2R alpha His-tag Protein, CF

Catalog # 10305-RL | R&D Systems, Inc. a Bio-Techne Brand
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10305-RL-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD25/IL-2R alpha protein
Glu22-Cys213, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

23 kDa

SDS-PAGE

35-43 kDa, under reducing conditions

Activity

Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.
The ED50 for this effect is 0.15-1.2 µg/mL in the presence of 30 ng/mL of Recombinant Human IL-2 (Catalog # 202-IL)

Scientific Data Images for Recombinant Human CD25/IL-2R alpha His-tag Protein, CF

Recombinant Human CD25/IL-2R alpha His-tag Protein Bioactivity

Recombinant Human CD25/IL-2R alpha His-tag Protein Bioactivity

Recombinant Human CD25/IL-2R alpha His-tag (Catalog # 10305-RL) inhibits IL-2 dependent cell proliferation of the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.15-1.2 μg/mL.
Recombinant Human CD25/IL-2R alpha His-tag Protein SDS-PAGE

Recombinant Human CD25/IL-2R alpha His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human CD25/IL-2R alpha His-tag (Catalog # 10305-RL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-43 kDa.

Formulation, Preparation and Storage

10305-RL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2R alpha), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit (gamma c). Human IL-2R alpha cDNA encodes a 213 amino acid (aa) precursor with a 21 aa signal peptide and a 192 aa extracellular region. The ECD of Human IL-2R alpha shares a 59% amino acid sequence identity with the ECD of mouse and rat IL-2R alpha, respectively. IL‑2R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). IL-2R beta (CD122) and gammac (IL-2R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (4, 5). By itself, IL-2R alpha binds IL-2 with low affinity. IL-2R alpha makes no contacts with IL-2R beta or gammac, and only minor changes are observed in the IL-2 structure in response to receptor binding. These findings support the principal role of IL-2R alpha to deliver IL-2 to the signaling complex and act as regulator of signal transduction (6, 7). A soluble form of IL‑2R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL‑2R alpha are found in some cancers and immune disorders (10). IL-2R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2R alpha is also required for the development of CD4+CD25+ Treg which suppresses autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11-13).

References

  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Kovanen, P.E. and Leonard, W.J. (2004) Immunol. Rev. 202:67.
  3. Bluestone, J.A. and Tang, Q. (2005) Curr. Opin. Immunol. 17:638.
  4. Hatakeyama, M. et al. (1989) Science 244:551.
  5. Takeshita, T. et al. (1992) Science 257:379.
  6. Stauber, D. et al. (2006) Proc. Natl. Acad. Sci. U. S. A. 103:2788.
  7. Wang, X. et al. (2005) Science 310:1159.
  8. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
  9. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
  10. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
  11. Willerford, D.M. et al. (1995) Immunity 3:521.
  12. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
  13. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Gene Symbol

IL2RA

UniProt

Additional CD25/IL-2R alpha Products

Product Documents for Recombinant Human CD25/IL-2R alpha His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD25/IL-2R alpha His-tag Protein, CF

For research use only

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FAQs for Recombinant Human CD25/IL-2R alpha His-tag Protein, CF

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  • Q: What are the differences between Recombinant Human CD25/IL-2R alpha Protein (Catalog # 223-2A) and Recombinant Human CD25/IL-2R alpha His-tag Protein, CF (Catalog # 10305-RL)?

    A: Catalog # 223-2A is expressed using the Mouse myeloma cell line, NS0, and is untagged.
    Catalog # 10305-RL is expressed using the Human embryonic kidney cell line, HEK293, and contains a C-terminal 6-His tag.

    The activity of these proteins is measured by their ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. Catalog # 223-2A has an ED50 of 0.15‑0.75 µg/mL in the presence of 30 ng/mL of recombinant human IL-2, while Catalog # 10305-RL has an ED50 of 0.15-1.2 µg/mL in the presence of 30 ng/mL of Recombinant Human IL-2.

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