Recombinant Human CCL3L1/LD78 beta
R&D Systems, part of Bio-Techne | Catalog # 509-MI
Key Product Details
- R&D Systems E. coli-derived Recombinant Human CCL3L1/LD78 beta (509-MI)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
E. coli-derived human CCL3L1/MIP-1 alpha Isoform LD78 beta protein
Ala24-Ala93
Ala24-Ala93
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala24
Predicted Molecular Mass
7.8 kDa
Activity
Measured by its ability to chemoattract 2-day cultured human monocytes.
The ED50 for this effect is 1-5 ng/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5.
The ED50 for this effect is 0.1-0.4 ng/mL.
The ED50 for this effect is 1-5 ng/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR5.
The ED50 for this effect is 0.1-0.4 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 509-MI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 509-MI/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL3L1/MIP-1 alpha Isoform LD78 beta
The macrophage inflammatory protein-1 alpha (MIP-1 alpha)/CCL3 and-1 beta (MIP-1 beta)/CCL4 are highly homologous CC family chemokines that were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Mouse MIP-1 alpha and MIP-1 beta are encoded by their respective single-copy genes. In human, additional non-allelic MIP-1 alpha and MIP-1 beta genes also exist (1‑3).
The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78 alpha and CCL3L1/LD78 beta, which share 94% amino acid (aa) sequence homology. Whereas the human CCL3/LD78 alpha is a single-copy gene, the human CCL3L1/LD78 beta gene copy number varies within the population. Human CCL3L1/LD78 beta cDNA encodes a 93 aa residue precursor with a 23 aa residue signal peptide that is cleaved to generate a 70 aa mature protein. Human CCL3L1/LD78 beta binds and signals through chemokine receptors CCR1 and CCR5. When compared to CCL3/LD78 alpha, CCL3L1/LD78 beta has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility (4).
References
- Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
- Nibbs, R.J. et al. (1999) J. Biol. Chem. 274:17478.
- Xin, X. et al. (1999) FEBS Lett. 457:219.
- Mackay, C.R. (2005) Trends in Molecular Medicine 11:203.
Alternate Names
G0S19-2, LD78 beta, MIP1 alpha Isoform LD78 beta, SCYA3L1
Entrez Gene IDs
414062 (Human)
Gene Symbol
CCL3L3
UniProt
Additional CCL3L1/MIP-1 alpha Isoform LD78 beta Products
Product Documents for Recombinant Human CCL3L1/LD78 beta
Product Specific Notices for Recombinant Human CCL3L1/LD78 beta
For research use only
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