Recombinant Human CCL21/6Ckine Protein

Name: Recombinant Human CCL21/6Ckine Protein
Brand: R&D Systems
SKU: 366-6C

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 366-6C

Citations (31)

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Key Product Details

Source:	E. coli
Accession #:	Q6ICR7
Applications:	Bioactivity

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Product Specifications for Recombinant Human CCL21/6Ckine Protein

Source	E. coli-derived human CCL21/6Ckine protein Ser24-Pro134
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser24
Predicted Molecular Mass	12 kDa
SDS-PAGE	16-17 kDa, reducing conditions
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED₅₀ for this effect is 3-15 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

366-6C

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

366-6C/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL21/6Ckine

CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a 12 kDa homeostatic chemokine that plays an important role in adaptive immune responses and inflammation (1). Unlike other CC chemokines, human CCL21 has a 37 amino acid (aa) C-terminal extension which mediates its attachment to carbohydrate structures and extracellular matrix components (2, 3). Mature human CCL21 shares 71% and 66% aa sequence identity with mouse and rat CCL21, respectively (4‑6). Both human and mouse CCL21 signal through the chemokine receptor CCR7, while mouse CCL21 additionally can signal through CXCR3 (7). CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC) (8‑10). Immobilized CCL21 promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation (8, 9). DC interaction with the anchored chemokine can induce CCL21 cleavage and release of an 8 kDa fragment that lacks the C-terminal extension (10). During chronic inflammation or tissue damage, CCL21 is up‑regulated on local vascular endothelial cells, macrophages, T cells, and neurons (11‑14). In these settings, it promotes fibrosis, inflammatory cytokine production, and neuropathic pain (12‑14). The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients (11, 13). CCL21 has been shown to exert either angiogenic or angiostatic effects (11, 15, 16). These effects, in combination with the ability of CCL21 to attract immune suppressor cells (Treg and MDSC) to a tumor site can have positive or negative effects on tumor progression (16, 17).

Item	Value
References	Forster, R. et al. (2008) Nat. Rev. Immunol. 8:362. Yang, B.G. et al. (2007) J. Immunol. 179:4376. Rey-Gallardo, A. et al. (2010) Glycobiology 20:1139. Hromas, R. et al. (1997) J. Immunol. 159:2554. Nagira, M. et al. (1997) J. Biol. Chem. 272:19518. Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589. Jenh, C. et al. (1999) J. Immunol. 162:3765. Tal, O. et al. (2011) J. Exp. Med. 208:2141. Friedman, R.S. et al. (2006) Nat. Immunol. 7:1101. Schumann, K. et al. (2010) Immunity 32:703. Pickens, S.R. et al. (2012) Arthritis Rheum. 64:2471. Sakai, N. et al. (2006) Proc. Natl. Acad. Sci. USA 103:14098. Damas, J.K. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:614. Biber, K. et al. (2011) EMBO J. 30:1864. Soto, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8205. Vicari, A.P. et al. (2000) J. Immunol. 165:1992. Shields, J.D. et al. (2010) Science 328:749.
Entrez Gene IDs	6366 (Human); 18829 (Mouse); 298006 (Rat)
Alternate Names	6Ckine, 6CkineSmall-inducible cytokine A21, Beta-chemokine exodus-2, CCL21, CKb9, SCYA21, SCYA21MGC34555, SLC, SLCSecondary lymphoid-tissue chemokine, TCA-4, chemokine (C-C motif) ligand 21, exodus-2, member 21, secondary lymphoid tissue chemokine

Citations for Recombinant Human CCL21/6Ckine Protein (31)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CCL21/6Ckine Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

EP Brandum et al. (2022), Selective Boosting of CCR7-Acting Chemokines; Short Peptides Boost Chemokines with Short Basic Tails, Longer Peptides Boost Chemokines with Long Basic Tails International Journal of Molecular Sciences, 23(3).
PMID: 35163323
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

FY Mao et al. (2021), Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense Cellular and Molecular Gastroenterology and Hepatology, 0(0).
PMID: 33676046
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

ML Capitano et al. (2021), A humanized monoclonal antibody against the endothelial chemokine CCL21 for the diagnosis and treatment of inflammatory bowel disease PLoS ONE, 16(7):e0252805.
PMID: 34197491
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Radaszkiew et al. (2021), RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy Elife, 10(0).
PMID: 34702444
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Kurowarabe et al. (2021), CXCL12-stimulated lymphocytes produce secondary stimulants that affect the surrounding cell chemotaxis Biochemistry and Biophysics Reports, 28(0):101128.
PMID: 34527817
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Dhandapani et al. (2021), Dendritic cells matured with recombinant human sperm associated antigen 9 (rhSPAG9) induce CD4+, CD8+ T cells and activate NK cells: a potential candidate molecule for immunotherapy in cervical cancer Cancer Cell International, 21(1):473.
PMID: 34493268
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Li et al. (2020), The lymph node stromal laminin alpha 5 shapes alloimmunity J. Clin. Invest., 0(0).
PMID: 32017712
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Zhang et al. (2020), A mutation that blocks integrin alpha4beta7 activation prevents adaptive immune-mediated colitis without increasing susceptibility to innate colitis BMC Biol., 18(1):64.
PMID: 32522281
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Ye et al. (2019), The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of dendritic cells by the TLR4 signaling pathway Cell Death Dis, 10(7):507.
PMID: 31263109
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

J Cantero-Pé et al. (2019), Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa Nat Commun, 10(1):4739.
PMID: 31628331
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SIA Mohamed et al. (2018), Dendritic cells pulsed with generated tumor cell lysate from Phyllanthus amarus Schum. & Thonn. induces anti-tumor immune response BMC Complement Altern Med, 18(1):232.
PMID: 30081891
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Caielli et al. (2018), A CD4+ T cell population expanded in lupus blood provides B cell help through interleukin-10 and succinate Nat. Med., 0(0).
PMID: 30478422
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JMJ Van den Be et al. (2017), Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use J Immunol Res, 2017(0):1975902.
PMID: 28785596
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

D Kobayashi et al. (2017), Regulation of CCR7-dependent cell migration throughï¿½CCR7 homodimer formation Sci Rep, 7(1):8536.
PMID: 28819198
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Alison Swaims-Koh (2016), Progesterone Levels Associate with a Novel Population of CCR5+CD38+ CD4 T Cells Resident in the Genital Mucosa with Lymphoid Trafficking Potential J Immunol, 197(1):368-76.
PMID: 27233960
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Till K et al. (2015), Expression of functional sphingosine-1 phosphate receptor-1 is reduced by B cell receptor signaling and increased by inhibition of PI3 kinase delta but not SYK or BTK in chronic lymphocytic leukemia cells. J Immunol, 194(5):2439-46.
PMID: 25632006
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Yu et al. (2015), Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway. PLoS ONE, 10(3):e0120855.
PMID: 25768730
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tutunea-Fatan et al. (2015), The role of CCL21/CCR7 chemokine axis in breast cancer-induced lymphangiogenesis. Mol Cancer, 14(1):35.
PMID: 25744065
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Borgman K et al. (2014), Priming by chemokines restricts lateral mobility of the adhesion receptor LFA-1 and restores adhesion to ICAM-1 nano-aggregates on human mature dendritic cells. PLoS ONE, 9(6):e99589.
PMID: 24945611
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Da Silva D et al. (2014), Suppression of Langerhans cell activation is conserved amongst human papillomavirus alpha and beta genotypes, but not a micro genotype. Virology, 452(0):279-86.
PMID: 24606705
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Irino et al. (2014), CC-Chemokine receptor CCR7: a key molecule for lymph node metastasis in esophageal squamous cell carcinoma. BMC Cancer, 14(0):291.
PMID: 24766770
Species: Human
Sample Types: Whole Cells
Applications: CAR-T (Bioassay)

Xu Y et al. (2014), Closely related T-memory stem cells correlate with in vivo expansion of CAR.CD19-T cells and are preserved by IL-7 and IL-15. Blood, 123(24):3750-9.
PMID: 24782509
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ehlin-Henriksson B et al. (2009), Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection. Immunology, 127(4):549-57.
PMID: 19604305
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Damas JK et al. (2009), Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication. Clin. Exp. Immunol., 157(3):400-7.
PMID: 19664149
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Berrih-Aknin S et al. (2009), CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia. Ann. Neurol., 66(4):521-31.
PMID: 19847900
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cagigi A et al. (2008), Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection. Blood, 112(12):4401-10.
PMID: 18780835
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hirahara K et al. (2006), The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors. J. Immunol., 177(7):4488-94.
PMID: 16982885
Species: Virus
Sample Types: Virus
Applications: Binding Assay

Nakayama T et al. (2006), Novel antiviral activity of chemokines. Virology, 350(2):484-92.
PMID: 16603217
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Curiel et al. (2004), Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival. Nat Med, 10(9):942-9.
PMID: 15322536
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Sen Y et al. (2004), Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 112(2):274-89.
PMID: 15147571

Recombinant Human CCL21/6Ckine Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human CCL21/6Ckine Protein

Formulation, Preparation and Storage

Carrier Free

366-6C

Carrier

366-6C/CF

Carrier Free

Reconstitution Calculator

Background: CCL21/6Ckine

Citations for Recombinant Human CCL21/6Ckine Protein (31)

Product Documents for Recombinant Human CCL21/6Ckine Protein

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