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Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF

Catalog # 2240-CA | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Accession #



Structure / Form

Disulfide-linked homodimer





Product Specifications


Mouse myeloma cell line, NS0-derived human Cadherin-12 protein
Human Pro-Cadherin-12
Accession # P55289
6-His tag
N-terminus C-terminus


>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln24 predicted

Predicted Molecular Mass

92.5 kDa (monomer)


115 kDa, reducing conditions


Measured by the ability of the immobilized protein to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells.
When 5 x 104 cells/well are added to Recombinant Human Pro-Cadherin‑12 Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately >30% will adhere after 30 minutes at 37 °C.
Optimal concentration depends on cell type as well as the application or research objectives.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate, NaCl and CaCl2.
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-12

The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1). Cadherin-12, also known as brain‑cadherin and N-cadherin 2, is a 150 kDa classical cadherin. Classical family molecules are modular in their extracellular region, mediating calcium‑dependent cell-cell adhesion through their five EC Ca++-binding repeats (2). Cadherin-12 can be further identified as a type II classical cadherin, due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat (3). Human Cadherin-12 is synthesized as a 794 amino acid (aa) type I transmembrane preproprotein that contains a 23 aa signal peptide, a 31 aa prosequence, a 555 aa extracellular region, a 28 aa transmembrane segment, and a 157 aa cytoplasmic domain (4, 5). The five EC cadherin domains are approximately 110 aa in length and generate two beta-sheets that are oriented like bread in a sandwich. Human Cadherin‑12 EC region is 96% aa identical to mouse Cadherin-12 EC region. Cadherin-12 is expressed specifically in CNS neurons. The bulk of its expression is postnatal, and it is proposed to be involved in synaptogenesis (4). As a classic cadherin, Cadherin-12 will form homodimers and promote intercellular adhesion with itself and, possibly, cadherins-8 and -14 (6).


  1. Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
  2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
  3. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136.
  4. Selig, S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2398.
  5. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
  6. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.

Alternate Names

Br-Cadherin, Cadherin12, CDH12, CDHB, N-Cadherin 2

Entrez Gene IDs

1010 (Human)

Gene Symbol



Product Documents for Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF

For research use only

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