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Recombinant Human C2GNT3/GCNT4 His-tag Protein

Bio-Techne includes R&D Systems | Catalog # 11162-GT

Glucosaminyl (N-acetyl) Transferase 4 Core 2
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11162-GT-020

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human C2GNT3/GCNT4 protein
Arg35-Ser453, with C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg35

Predicted Molecular Mass

49.7 kDa

SDS-PAGE

50-66 kDa, under reducing conditions.

Activity

Measured by its ability to transfer GlcNAc from UDP-GlcNAc to B1-3 galactosyl-N-acetyl galactosamine.
The specific activity is >75 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human C2GNT3/GCNT4 His-tag Protein

Recombinant Human C2GNT3/GCNT4 His-tag Protein/GCNT4 Enzyme Activity Diagram

GCNT4 (Catalog # 11162-GT) recognizes core-1 O-glycan and convert it to core-2 type O-glycan.

Recombinant Human C2GNT3/GCNT4 His-tag Protein SDS-PAGE

1 μg/lane of Recombinant Human GCNT4 His-tag (Catalog # 11162-GT ) was resolved with SDS-PAGE under reducing conditions and visualized by silver staining, showing a band at 50-66 kDa.

Formulation, Preparation and Storage

11162-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: C2GNT3/GCNT4

Mucin-type O-glycans are initiated with an O-GalNAc attachment to a serine or threonine in a polypeptide. The O-GalNAc residues are subsequently extended by various glycosyltransferases resulting in different types of O-glycans. Most O-glycans contain the core 1 structure, Gal beta1-3GalNAc. GCNT4 is a member of the glucosaminyl (N-acetyl) transferase family and it converts the core 1 O-glycan to core 2 O-glycan, Gal beta1-3(GlcNAc beta1-6)GalNAc, via the addition of a GlcNAc residue (1). Various ligand carbohydrates can be formed from core 2 branched oligosaccharides. For example, sialyl Lex in mucin‑type glycoproteins of blood cells can be formed from core 2 branched oligosaccharides (2,3). GCNT4 does not have core 4 O-glycan or I-branching enzyme activity. GCNT4 has been shown to play a role in gastric cancers, where GCNT4 is downregulated and its overexpression prevents cancer cell growth (4, 5). The activity of the recombinant human GCNT-4 was determined using a phosphatase‑coupled glycosyltransferase assay (6).

References

  1. Schwientek, T. et al. (2000) J Biol Chem. 275:11106.
  2. Hemmerich, S. et al. (1995) J. Biol. Chem. 270:12035.
  3. Wilkins, P.P. et al. (1996) J. Biol. Chem. 270:18732.
  4. Sun, H. et al. (2020) Onco. Targets Ther. 25:8601.
  5. Hu, W. et al. (2021) Bioengineered 12:11634.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

Glucosaminyl (N-acetyl) Transferase 4 Core 2

Alternate Names

GCNT4, Gm279, Gm73

Entrez Gene IDs

51301 (Human); 218476 (Mouse); 310011 (Rat)

Gene Symbol

GCNT4

UniProt

Additional C2GNT3/GCNT4 Products

Product Documents for Recombinant Human C2GNT3/GCNT4 His-tag Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human C2GNT3/GCNT4 His-tag Protein

For research use only

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