E. coli-derived human c-Abl protein Gly227-Gln513, with an N-terminal Met and 6-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
33-35 kDa, under reducing conditions
Measured by its ability to phosphorylate the Abl peptide substrate EAIYAAPFAKKK. The specific activity is >200 pmol/min/μg, as measured under the described conditions.
Recombinant Human c-Abl His-tag Protein, CF Scientific Data Examples
Recombinant Human c‑Abl His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human c‑Abl His-tag Protein (Catalog # 11091-AL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~33-35 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP and Glycerol.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Tyrosine protein kinase ABL1 (Abelson murine leukemia viral oncogene homolog 1) is a ubiquitously expressed, magnesium-dependent, cytosolic member of the ABL subfamily of non-receptor protein tyrosine kinases (1). Human nonreceptor tyrosine kinases share a conserved domain structure that contributes to activity regulation and substrate specificity in many key processes linked to cell growth and survival (2-4). In addition, ABL1 contains domains unique to the ABL1 paralog that enable its function in DNA binding and DNA-damage response. ABL1 is composed of an N-terminal glycine myristoyl group that blocks the surface pocket of the kinase domain, a cap that stabilizes an inactive conformation, SH3 and SH2 domains that impose a locked inactive state, a protein kinase region, three nuclear localization signals, a DNA-binding region, and C-terminal nuclear export signal motifs, and actin F-binding domain (5-9). Activity is regulated through disruption of autoinhibitory interactions and several phosphorylation events that increase or decrease activity or promote function through stabilization (4). The SH2 domain contributes to both catalytic activity and target site specificity as has been shown with domain swapping (10). In chronic myelogenous, acute myeloid, and acute lymphoblastic leukemias, ABL1 is fused to BCR gene, resulting in deletion of the regulatory domains and production of a constitutively active tyrosine kinase (11). Several mutations in ABL1 lead to misregulation of activity in a variety of cancers (12). ABL1 kinase inhibition is used for therapeutic treatment in leukemic cancers and requires further development of drugs to address ABL1-mutation conferred resistance (12,13).
Sefton, B.M. et al. (1981) Proc. Natl. Acad. Sci. USA 78:1552.
Bradley, W.D. and A.J. Koleske (2009) J. Cell Sci. 122:3441.
Gu, J.J. et al. (2009) Immunol. Rev. 228:170.
Colicelli, J. (2010) Sci. Signal. 14:139.
Wen, S.T. et al. (1996) EMBO J. 15:1853.
Taagepera, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:7457.
Pluk, H. et al. (2002) Cell. 108:247.
Hantschel, O. et al. (2003) Cell. 112:845.
Nagar, B. et al. (2006) Mol. Cell. 21:787.
Filippakopoulos, P. et al. (2008) Cell. 134:793.
Shtivelman, E. et al. (1986) Cell. 47:277.
Wang, J. and A.M. Pendergast. (2015) Trends Cancer 1:110.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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