To which isoform does the sequence of the Recombinant Human BTLA His-tag Protein, CF (Catalog # 9235-BT) correspond?

Name: Recombinant Human BTLA His-tag Protein, CF
Brand: R&D Systems
SKU: 9235-BT

The sequence of this protein is Lys31-Leu150 with a C-terminal 6-His tag. This sequence corresponds to Isoform 2 of Human BTLA based on Accession # Q7Z6A9-2, which is an alternative splicing variant of the canonical sequence that is Isoform 1.

Recombinant Human BTLA His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9235-BT

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Key Product Details

Source:	HEK293
Accession #:	NP_001078826.1
Applications:	Bioactivity

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Product Specifications for Recombinant Human BTLA His-tag Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human BTLA protein Lys31-Leu150, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Lys31
Predicted Molecular Mass	15 kDa
SDS-PAGE	23-33 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Human BTLA that produces 50% of the optimal binding response is approximately 0.1-0.5 μg/mL.

Scientific Data Examples for Recombinant Human BTLA His-tag Protein, CF

	Recombinant Human BTLA His-tag Protein Bioactivity When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is coated at 0.5 µg/mL, Recombinant BTLA (Catalog # 9235-BT) binds with an ED₅₀ of 0.1-0.5 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9235-BT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: BTLA

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules (1-3). Mature human BTLA contains a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane sequence, and a 111 aa cytoplasmic domain. The two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail transmit inhibitory signaling (4-5). The ECD of human BTLA shares 42% and 44% aa identity with that of mouse and rat BTLA, respectively. A splice variant lacking the transmembrane domain has been reported (6). Unlike other CD28 family members, the BTLA Ig domain in the ECD is of the I-type rather than V-type, and BTLA does not form homodimers (7). BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands (8). BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and NK cells (9). Its expression is low in naïve T cells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature naïve cells (9). BTLA apparently limits T cell numbers, since its deletion results in overproduction of T cells, especially CD8⁺ memory T cells that are hyper-responsive to TCR crosslinking (10). Under the control of ROR gamma t and IL-7, BTLA regulates the homeostasis and inflammatory responses of gamma δT cells (11). The binding of BTLA and HVEM does not preclude the concurrent binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha (12).

Item	Value
References	Murphy, K.M. et al. (2006) Nat. Rev. Immunol. 6:671. Croft, M. (2005) Trends. Immunol. 26:292. Watanabe, N. et al. (2003) Nat. Immunol. 4:670. Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236. Chemnitz, J.M. et al. (2006) J. Immunol. 176:6603. Han, P. et al. (2004) J. Immunol. 172 :5931 Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90. Hurchla, M.A. et al. (2005) J. Immunol. 174:3377. Krieg, C. et al. (2007) Nat. Immunol. 8:162. Bekiaris, V. et al. (2013) Immunity 39:1082. Cai G and Freeman GJ, (2009) Immunol Rev. 229:244
Long Name	B- And T-Lymphocyte Attenuator
Entrez Gene IDs	151888 (Human); 208154 (Mouse); 102144877 (Cynomolgus Monkey)
Alternate Names	B and T lymphocyte associated, B and T lymphocyte attenuator, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, BTLA, BTLA1, CD272, CD272 antigen, FLJ16065, MGC129743

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FAQs for Recombinant Human BTLA His-tag Protein, CF

To which isoform does the sequence of the Recombinant Human BTLA His-tag Protein, CF (Catalog # 9235-BT) correspond?

The sequence of this protein is Lys31-Leu150 with a C-terminal 6-His tag. This sequence corresponds to Isoform 2 of Human BTLA based on Accession # Q7Z6A9-2, which is an alternative splicing variant of the canonical sequence that is Isoform 1.