Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein

Name: Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein
Brand: R&D Systems
SKU: 315-BR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 315-BR

Citations (11)

Product Datasheet / COA / SDS

New Version Available - 2406-BR

A New rh BMPR-IA is Available! ~2 fold better activity; Lower endotoxin specification; Enhanced purity!

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	CAA80257
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein

Product Specifications for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein

Source

Mouse myeloma cell line, NS0-derived human BMPR-IA/ALK-3 protein

Human BMPR-IA (Gln24 - Arg152) Accession # CAA80257	IEGRMD	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

No results obtained: Gln24 predicted

Predicted Molecular Mass

41.5 kDa (monomer)

SDS-PAGE

55 kDa, reducing conditions

Activity

Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 0.1‑0.3 µg/mL in the presence of 30 ng/mL of recombinant human BMP-4.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

315-BR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

315-BR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMPR-IA/ALK-3

Bone Morphogenetic Protein Receptor IA (BMPR-IA), also known as ALK-3, BRK-1, and CD292, is a glycosylated 60 - 65 kDa type I receptor in the TGF‑ beta serine/threonine kinase receptor family (1 ‑ 3). Binding of TGF-beta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Mature human BMPR-IA consisits of a 129 amino acid (aa) extracellular domain (ECD), a 24 aa transmembrane segment, and a 356 aa cytoplasmic region that contains the tyrosine kinase domain (4). Within the ECD, human BMPR-IA shares 98% and 97% aa sequence identity with mouse and rat BMPR-IA, respectively. BMPR-IA is involved in the development and function of a wide range of tissues. During early embryogenesis it is required for migration of the anterior visceral endoderm (AVE) and proper development of the anterior-posterior axis (5). Tissue-specific conditional knockout experiments have demonstrated the importance of BMPR-IA in the development and morphogenesis of the heart, lung, palate, teeth, and mandible (6 - 8). In the adult, BMPR-IA plays a role in glucose-stimulated insulin secretion by pancreatic beta cells, osteoclast activity and bone remodeling, reactive astrocyte-mediated scar formation following spinal cord injury, and ovulation and fertility (9 - 12).

Item	Value
References	Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1. ten Dijke, P. et al. (1993) Oncogene 8:2879. Miura, S. et al. (2010) Dev. Biol. 341:246. Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878. Sun, J. et al. (2008) Am. J. Pathol. 172:571. Li, L. et al. (2011) Dev. Biol. 349:451. Goulley, J. et al. (2007) Cell Metab. 5:207. Kamiya, N. et al. (2008) J. Bone Miner. Res. 23:2007. Sahni, V. et al. (2010) J. Neurosci. 30:1839. Edson, M.A. et al. (2010) Mol. Endocrinol. 24:1251.
Long Name	Bone Morphogenetic Protein Receptor IA/Activin Receptor-like Kinase 3
Entrez Gene IDs	657 (Human); 12166 (Mouse)
Alternate Names	ACVRLK310q23del, ALK-3, ALK3EC 2.7.11.30, BMP type-1A receptor, BMPR-1A, BMPR-IA, BMPR1A, BMPRIA, CD292, CD292 antigen, EC 2.7.11, SKR5, Serine/threonine-protein kinase receptor R5, bone morphogenetic protein receptor type-1A, bone morphogenetic protein receptor, type IA, type II-like kinase 3

Citations for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein (11)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein include:

Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

T Mang et al. (2020), BMPR1A is necessary for chondrogenesis and osteogenesis while BMPR1B prevents hypertrophic differentiation J. Cell. Sci., 0(0).
PMID: 32764110
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Woltje K et al. (2015), Serum induces transcription of Hey1 and Hey2 genes by Alk1 but not Notch signaling in endothelial cells. PLoS ONE, 10(3):e0120547.
PMID: 25799559
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Martinez V et al. (2015), The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis. PLoS ONE, 10(6):e0131453.
PMID: 26110906
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Castonguay R et al. (2011), Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 286(34):30034-46.
PMID: 21737454
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Steinbicker AU et al. (2011), Inhibition of bone morphogenetic protein signaling attenuates anemia associated with inflammation. Blood, 117(18):4915-23.
PMID: 21393479
Sengle G et al. (2008), A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. J. Mol. Biol., 381(4):1025-39.
PMID: 18621057
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Jeanpierre S et al. (2008), BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling. Blood, 112(8):3154-63.
PMID: 18664625
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

David L et al. (2007), Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells. Blood, 109(5):1953-61.
PMID: 17068149
Species: Human
Sample Types: Recombinant Protein
Applications: Receptor Binding Assay

Reversade B et al. (2005), Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field. Cell, 123(6):1147-60.
PMID: 16360041
Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay

Gunhaga L et al. (2003), Specification of dorsal telencephalic character by sequential Wnt and FGF signaling. Nat. Neurosci., 6(7):701-7.
PMID: 12766771
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Bigley V et al. (0), Langerin-expressing dendritic cells in human tissues are related to CD1c+ dendritic cells and distinct from Langerhans cells and CD141high XCR1+ dendritic cells. J Leukoc Bio97(4):627-34.
PMID: 25516751

Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein

Formulation, Preparation and Storage

Carrier Free

315-BR

Carrier

315-BR/CF

Carrier Free

Reconstitution Calculator

Background: BMPR-IA/ALK-3

Citations for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein (11)

Product Documents for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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