What is the half-life of a recombinant or natural protein in culture at 37 °C?

Name: Recombinant Human BMP-2/BMP-7 Heterodimer Protein
Brand: R&D Systems
SKU: 3229-BM

R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

Recombinant Human BMP-2/BMP-7 Heterodimer Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3229-BM

Citations (17)

Product Datasheet / COA / SDS

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Key Product Details

Source:	E. coli
Accession #:	NP_001191 (BMP-2) & NP_001710 (BMP-7)
Structure / Form:	Disulfide-linked heterodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human BMP-2/BMP-7 Heterodimer Protein

Product Specifications for Recombinant Human BMP-2/BMP-7 Heterodimer Protein

Source

E. coli-derived human BMP-2/BMP-7 Heterodimer protein

Human BMP-2
(Ala284 - Arg396)
Accession # NP_001191

Human BMP-7 (Ser293 - His431) Accession # NP_001710
N-terminus	C-terminus

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala284 & Ser293

Predicted Molecular Mass

12.9 kDa (BMP-2), 15.8 kDa (BMP-7)

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 15.0-120 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3229-BM

Carrier

Formulation	Supplied as 0.2 μm filtered solution in 30%(v/v) Acetonitrile and 0.1%(v/v) TFA with 50 μg BSA per 1 μg as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

3229-BM/CF

Carrier Free

Formulation	Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-2/BMP-7 Heterodimer

Human BMP-7, also known as osteogenic protein 1 (OP-1), and BMP-2 are members of the BMP subgroup of the TGF-beta superfamily and signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-2 and BMP-7 influence a variety of morphogenic processes, particularly during skeletal and renal development (1 - 3). The human BMP-2 cDNA encodes a 396 amino acid (aa) precursor that contains a 23 aa signal sequence, a 259 propeptide, and a 114 aa mature protein (4). The human BMP-7 cDNA encodes a 431 aa precursor that contains a 29 aa signal sequence, a 263 aa propeptide, and a 139 aa mature protein (5). BMP propeptides are removed by proteolysis, enabling mature BMPs to form active disulfide linked homodimers or heterodimers (1). Human and mouse BMP-2 and BMP-7 are 100% and 98% identical, respectively, at the amino acid level. Human BMP-2 shares 85% aa sequence identity with human BMP-4 and less than 51% aa sequence identity with other BMPs. Human BMP-7 shares approximately 60% - 70% aa sequence identity with BMP-5, -6, and -8, and less than 50% aa sequence identity with other BMPs. BMP-2 and BMP-7 are co-expressed in some embryonic tissues (6, 7) and associate into a functional 38 kDa osteogenic dimer (8). In in vitro osteoblast differentiation assays and in vivo bone formation models, a BMP-2/BMP-7 heterodimer is significantly more potent than either homodimer (9 - 12). Considering that BMP-2 preferentially binds BMPRIA/ALK-3 and BMPRIB/ALK-6, while BMP-7 is selective for ALK-2, the observed increase in heterodimer activity may be due to the triggering of additional receptor subtypes.

Item	Value
References	Chen, D. et al. (2004) Growth Factors 22:233. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299. Wozney, J. et al. (1988) Science 242:1528. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970. Lyons, K.M. et al. (1995) Mech. Dev. 50:71. Knosp, W.M. et al. (2004) Development 131:4581. Sampath, T.K. et al. (1990) J. Biol. Chem. 265:13198. Hazama, M. et al. (1995) Biochem. Biophys. Res. Commun. 209:859. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021. Zhao, M. et al. (2005) J. Cell. Biochem. 95:1. Israel, D.I. et al. (1996) Growth Fact ors 13:291.
Long Name	Bone Morphogenetic Protein 2/7
Entrez Gene IDs	650 (Human)
Alternate Names	BMP-2/BMP-7 Heterodimer

Citations for Recombinant Human BMP-2/BMP-7 Heterodimer Protein (17)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human BMP-2/BMP-7 Heterodimer Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

YS Yang et al. (2022), Suppression of heterotopic ossification in fibrodysplasia ossificans progressiva using AAV gene delivery Oncogene, 13(1):6175.
PMID: 36258013
Species: Zebrafish
Sample Types: Embryo
Applications: Bioassay

H Greenfeld et al. (2021), The BMP signaling gradient is interpreted through concentration thresholds in dorsal-ventral axial patterning PloS Biology, 19(1):e3001059.
PMID: 33481775
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

JM Kim et al. (2020), A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation Nat Commun, 11(1):2289.
PMID: 32385263
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

A Krouwels et al. (2020), Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration Int J Mol Sci, 21(8).
PMID: 32295299
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SEJ Tanis et al. (2019), BLNCR is a long non-coding RNA adjacent to integrin beta-1 that is rapidly lost during epidermal progenitor cell differentiation Sci Rep, 9(1):31.
PMID: 30631104
Species: Chicken
Sample Types: Tissue Explants
Applications: Bioassay

WKW Ho et al. (2019), Feather arrays are patterned by interacting signalling and cell density waves PLoS Biol., 17(2):e3000132.
PMID: 30789897
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AM El-Naggar et al. (2019), HACE1 is a potential tumor suppressor in osteosarcoma Cell Death Dis, 10(1):21.
PMID: 30622235
Applications: ELISA (Standard)

LD Loozen et al. (2018), Bone formation by heterodimers through non-viral gene delivery of BMP-2/6 and BMP-2/7 Eur Cell Mater, 35(0):195-208.
PMID: 29589649
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

W Bi et al. (2018), All-trans retinoic-acid inhibits heterodimeric bone morphogenetic protein 2/7-stimulated osteoclastogenesis, and resorption activity Cell Biosci, 8(0):48.
PMID: 30159139
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SEJ Tanis et al. (2018), Splicing and Chromatin Factors Jointly Regulate Epidermal Differentiation Cell Rep, 25(5):1292-1303.e5.
PMID: 30380419
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

P Choudhary et al. (2017), Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage Stem Cells Transl Med, 6(2):490-501.
PMID: 28191760
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Greenblatt M et al. (2015), p38alpha MAPK is required for tooth morphogenesis and enamel secretion. J Biol Chem, 290(1):284-95.
PMID: 25406311
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ochiai-Shino H et al. (2014), A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination. PLoS ONE, 9(6):e99534.
PMID: 24911063
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Goldie S et al. (2012), FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis. Cancer Res, 72(13):3424-36.
PMID: 22564525
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Castonguay R et al. (2011), Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 286(34):30034-46.
PMID: 21737454
Species: Zebrafish
Sample Types: Whole Tissue
Applications: Bioassay

Little SC et al. (2009), Bone morphogenetic protein heterodimers assemble heteromeric type I receptor complexes to pattern the dorsoventral axis. Nat. Cell Biol., 11(5):637-43.
PMID: 19377468
Species: Zebrafish
Sample Types: In Vivo
Applications: In Vivo

Esterberg R et al. (2008), Tailbud-derived Bmp4 drives proliferation and inhibits maturation of zebrafish chordamesoderm. Development, 135(23):3891-901.
PMID: 18948415

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Showing 1 - 10 of 17 citations Showing All

FAQs for Recombinant Human BMP-2/BMP-7 Heterodimer Protein

What is the half-life of a recombinant or natural protein in culture at 37 °C?

R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

Recombinant Human BMP-2/BMP-7 Heterodimer Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human BMP-2/BMP-7 Heterodimer Protein

Formulation, Preparation and Storage

Carrier Free

3229-BM

Carrier

3229-BM/CF

Carrier Free

Reconstitution Calculator

Background: BMP-2/BMP-7 Heterodimer

Citations for Recombinant Human BMP-2/BMP-7 Heterodimer Protein (17)

FAQs for Recombinant Human BMP-2/BMP-7 Heterodimer Protein

Product Documents for Recombinant Human BMP-2/BMP-7 Heterodimer Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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