Recombinant Human B7-H3 Fc Chimera Protein, CF

Name: Recombinant Human B7-H3 Fc Chimera Protein, CF
Brand: rnd
SKU: 1027-B3
Rating: 5 (1 reviews)

Catalog # 1027-B3

Citations (3)

(1)

Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	NP_079516
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications

Source

Mouse myeloma cell line, NS0-derived human B7-H3 protein

Human B7-H3 (Leu29-Pro245) Accession # NP_079516	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu29

Predicted Molecular Mass

50 kDa (monomer)

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED₅₀ for this effect is 3-12 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1027-B3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H3

Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for regulating immune responses (1‑3). Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% amino acid (aa) sequence identity. The cloned human B7-H3 cDNA encodes a 316 aa type I membrane precursor protein with a putative 28 aa signal peptide, a 217 aa extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a 45 aa cytoplasmic domain. An isoform of human B7-H3 containing a four-Ig-like domain extracellular region has also been identified. Human B7-H3 is not expressed on resting B cells, T cells, monocytes or dendritic cells, but is induced on dendritic cells and monocytes by inflammatory cytokines. B7-H3 expression is also detected on various normal tissues and in some tumor cell lines. Human B7-H3 does not bind any known members of the CD28 family of immunoreceptors. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to costimulate the proliferation of CD4⁺ or CD8⁺ T cells. B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production (1-3).

Item	Value
References	Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Long Name	B7 Homolog 3
Entrez Gene IDs	80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)
Alternate Names	B7-H3, B7-H3B7 homolog 3, B7H3, B7H34Ig-B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule

Citations (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human B7-H3 Fc Chimera Protein, CF include:

Applications: ELISA (Standard)

UM Vogl et al. (2017), Evaluation of Prognostic Immune Signatures in Patients with Breast, Colorectal and Pancreatic Cancer Receiving Chemotherapy Anticancer Res., 37(4):1947-1955.
PMID: 28373465
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

BR Yoon et al. (2016), Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis J. Biol. Chem, 291(8):4048-57.
PMID: 26702052
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Zhang G et al. (2010), B7-h3 augments the inflammatory response and is associated with human sepsis. J. Immunol., 185(6):3677-84.
PMID: 20696859

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Recombinant Human B7-H3 Fc Chimera Protein, CF

Name: Anonymous

Application: Enzymatic activity in vitro

Reason for Rating: Worked perfectly in ELISA analysis of antibody production of cell fusions.

Verified Customer | Posted 12/01/2017

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Product Datasheets

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Recombinant Human B7-H3 Fc Chimera Protein, CF

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Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: B7-H3

Citations (3)

Customer Reviews (1)

Recombinant Human B7-H3 Fc Chimera Protein, CF

Product Datasheets

Product Datasheets

COA

Certificate of Analysis

SDS

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