Recombinant Human B7-H3 (4Ig)/B7-H3b Protein Best Seller

Catalog # 2318-B3 | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
2318-B3-01M/CF
2318-B3-050/CF
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With Carrier
2318-B3-050
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Key Product Details

Accession #

Q5ZPR3

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human B7-H3 protein
Gly27-Thr461, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly27

Predicted Molecular Mass

48 kDa

SDS-PAGE

78-90 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and/or 10 µg/mL rhB7-H3 (4Ig)/B7-H3b.
The presence of rhB7-H3 (4Ig)/B7-H3b at 10 µg/mL inhibited the anti-CD3 response by >30%.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2318-B3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2318-B3/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H3

Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses (1 - 3). Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Among the family members there is about 20 - 40% amino acid (aa) sequence identity. B7-H3 was initially reported to be a 316 aa type I transmembrane precursor protein that contained a signal sequence, an extracellular region with one V‑type and one C‑type Ig domain, a transmembrane segment and a short cytoplasmic tail (1). Subsequent studies have identified a second 110 kDa form whose precursor is 534 aa in length. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern (4, 5). It would appear that the human 4Ig form is the principal, if not the only form of B7-H3 (5). Its precursor contains a 26 aa signal sequence, a 435 aa extracellular region, a 31 aa transmembrane domain, and a 42 aa cytoplasmic tail. The four Ig-like domains alternate between V‑type and C‑type, and apparently are the consequence of a V‑C type tandem duplication (4, 5). B7-H3b is expressed on dendritic cells as well as activated T, B and NK cells (5). The mouse gene differs from that of human in that it cannot code for four Ig-like domains; only a V‑type:C‑type pair (4). Human B7-H3b binding to an undefined receptor has shown to be inhibitory to NK cell illing and cytokine release (6). It also seems to be required for late stage osteoblast differentiation (7).

References

  1. Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269.
  2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J.Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Sun, M. et al. (2002) J. Immunol. 168:6294.
  5. Steinberger, P. et al. (2004) J. Immunol. 172:2352.
  6. Prasad, D.V.R. et al. (2004) J. Immunol. 173:2500.
  7. Suh, W-K. et al. (2004) Proc. Natl. Acad. Sci. USA 101:12969.

Long Name

B7 Homolog 3

Alternate Names

B7H3, CD276

Entrez Gene IDs

80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)

Gene Symbol

CD276

UniProt

Q5ZPR3

Product Documents for Recombinant Human B7-H3 (4Ig)/B7-H3b Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 2318-B3 (with carrier)
Download SDS 2318-B3/CF (carrier free)

Product Specific Notices for Recombinant Human B7-H3 (4Ig)/B7-H3b Protein

For research use only

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Customer Reviews for Recombinant Human B7-H3 (4Ig)/B7-H3b Protein (3)

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  • Recombinant Human B7-H3 (4Ig)/B7-H3b Protein
    Name: Donghyun Kim
    Application: SDS-PAGE Control
    Verified Customer | Posted 01/10/2020
  • Recombinant Human B7-H3 (4Ig)/B7-H3b Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/10/2020
  • Recombinant Human B7-H3 (4Ig)/B7-H3b Protein
    Name: Jenna Nguyen
    Application: SDS-PAGE Control
    Verified Customer | Posted 05/07/2019

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