Recombinant Human B7-H3 (4Ig)/B7-H3b Protein Best Seller
Catalog # 2318-B3 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Gly27-Thr461, with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and/or 10 µg/mL rhB7-H3 (4Ig)/B7-H3b.
The presence of rhB7-H3 (4Ig)/B7-H3b at 10 µg/mL inhibited the anti-CD3 response by >30%.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 2318-B3
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 2318-B3/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: B7-H3
Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses (1 - 3). Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Among the family members there is about 20 - 40% amino acid (aa) sequence identity. B7-H3 was initially reported to be a 316 aa type I transmembrane precursor protein that contained a signal sequence, an extracellular region with one V‑type and one C‑type Ig domain, a transmembrane segment and a short cytoplasmic tail (1). Subsequent studies have identified a second 110 kDa form whose precursor is 534 aa in length. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern (4, 5). It would appear that the human 4Ig form is the principal, if not the only form of B7-H3 (5). Its precursor contains a 26 aa signal sequence, a 435 aa extracellular region, a 31 aa transmembrane domain, and a 42 aa cytoplasmic tail. The four Ig-like domains alternate between V‑type and C‑type, and apparently are the consequence of a V‑C type tandem duplication (4, 5). B7-H3b is expressed on dendritic cells as well as activated T, B and NK cells (5). The mouse gene differs from that of human in that it cannot code for four Ig-like domains; only a V‑type:C‑type pair (4). Human B7-H3b binding to an undefined receptor has shown to be inhibitory to NK cell illing and cytokine release (6). It also seems to be required for late stage osteoblast differentiation (7).
References
- Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269.
- Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
- Coyle, A. and J.Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Sun, M. et al. (2002) J. Immunol. 168:6294.
- Steinberger, P. et al. (2004) J. Immunol. 172:2352.
- Prasad, D.V.R. et al. (2004) J. Immunol. 173:2500.
- Suh, W-K. et al. (2004) Proc. Natl. Acad. Sci. USA 101:12969.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human B7-H3 (4Ig)/B7-H3b Protein
Product Specific Notices for Recombinant Human B7-H3 (4Ig)/B7-H3b Protein
For research use only
Citations for Recombinant Human B7-H3 (4Ig)/B7-H3b Protein (2)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human B7-H3 (4Ig)/B7-H3b Protein include:
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Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA CaptureH Li et al. (2022-02-03), LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma Cell Death & Disease, 2022-013(2):109.
PMID: 35115487 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayBR Yoon et al. (2015-12-23), Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis J. Biol. Chem, 2015-1291(8):4048-57.
PMID: 26702052
There are no citations that match your criteria.
Customer Reviews for Recombinant Human B7-H3 (4Ig)/B7-H3b Protein (3)
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Application: SDS-PAGE ControlVerified Customer | Posted 01/10/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 01/10/2020
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Application: SDS-PAGE ControlVerified Customer | Posted 05/07/2019
There are no reviews that match your criteria.
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