Recombinant Human B7-H2 Isoform 3 Fc Chimera Protein, CF

Name: Recombinant Human B7-H2 Isoform 3 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10028-B7

Catalog # 10028-B7

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	O75144-3
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human B7-H2 Isoform 3 Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human B7-H2 protein

Human B7-H2 Isoform 3 (Ala19-Thr139) Accession # O75144-3	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala19

Predicted Molecular Mass

40 kDa

SDS-PAGE

57-66 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human ICOS Fc Chimera is immobilized at 5 µg/mL, Recombinant Human B7‑H2 Isoform 3 Fc Chimera binds with an ED₅₀ of 10-50 μg/mL.

Recombinant Human B7-H2 Isoform 3 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human B7-H2 Isoform 3 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human B7‑H2 Isoform 3 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-67 kDa and 110-130 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10028-B7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H2

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H2 consists of a 238 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like (Ig-like) domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain (2, 3). Within the ECD, human B7-H2 shares 50% and 54% aa sequence identity with mouse and rat B7‑H2, respectively. Alternative splicing generates a long isoform (Isoform 2), which carries a 10 aa substitution for the C-terminal 3 residues, and a short isoform (Isoform 3; this product), which lacks the first Ig-like domain (aa 18 - 134 is missing). B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells (2-6). It binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation (2, 4, 7). Mouse and human B7-H2 exhibit cross-species binding to ICOS (3, 6). The B7-H2 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (T^FH) and germinal centers (2-4, 8, 9). In human but not in mouse, B7-H2 additionally binds to CD28 and CTLA4, and its interaction with CD28 can costimulate both human and mouse naïve T cells and regulatory T cells (Treg) (6). B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung (8, 10, 11). Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity (5, 12, 13). In contrast, its ligation of ICOS on T^FH cells can increase the severity of autoimmune symptoms (9). A soluble form of human B7-H2 is elevated in the circulation of patients with active systemic lupus erythematosus (14). In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thryoid hormones (15).

Item	Value
References	Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180. Wang, S. et al. (2000) Blood 96:2808. Yoshinaga, S.K. et al. (2000) Int. Immunol. 12:1439. Yoshinaga, S.K. et al. (1999) Nature 402:827. Faget, J. et al. (2012) Cancer Res. 72:6130. Yao, S. et al. (2011) Immunity 32:729. Watanabe, M. et al. (2008) J. Immunol. 180:5222. Wong, S.-C. et al. (2003) Blood 102:1381. Hu, Y.-L. et al. (2009) J. Immunol. 182:1421. Kadkhoda, K. et al. (2010) J. Immunol. 184:3780. Kadkhoda, K. et al. (2011) Int. Immunol. 23:239. Gajewska, B.U. et al. (2005) J. Immunol. 174:3000. Akbari, O. et al. (2002) Nat. Med. 8:1024. Her, M. et al. (2009) Lupus 18:501. Wang, F. et al. (2012) J. Clin. Immunol. 32:1253.
Long Name	B7 Homolog 2
Entrez Gene IDs	23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)
Alternate Names	B7-H2, B7-H2B7-related protein 1, B7H2, B7H2B7 homolog 2, B7RP-1, B7RP-1LICOS, B7RP1B7-like protein Gl50, CD275, CD275 antigen, GL50, GL50transmembrane protein B7-H2 ICOS ligand, ICOS-L, ICOSL, ICOSLG, KIAA0653ICOS ligand, inducible T-cell co-stimulator ligand

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