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Recombinant Human ASGR1/ASGPR1 Fc Chimera Protein, CF

Catalog # 10255-AS | R&D Systems, Inc. a Bio-Techne Brand
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10255-AS-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ASGR1/ASGPR1 protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human ASGR1
(Asn63-Leu291)
Accession # P07306-1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

53 kDa

SDS-PAGE

59-71 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human ASGR1/ASGPR1 Fc Chimera (Catalog # 10255-AS) binds human plasma von Willebrand Factor with an ED50 of 0.15-0.9 μg/mL.

Scientific Data Images for Recombinant Human ASGR1/ASGPR1 Fc Chimera Protein, CF

Recombinant Human ASGR1/ASGPR1 Fc Chimera Protein Bioactivity

Recombinant Human ASGR1/ASGPR1 Fc Chimera Protein Bioactivity

Immobilized Recombinant Human ASGR-1/ASGPR1 Fc Chimera (Catalog # 10255-AS) binds human plasma von Willebrand Factor with an ED50 of 150-900 ng/mL.
Recombinant Human ASGR1/ASGPR1 Fc Chimera Protein SDS-PAGE

Recombinant Human ASGR1/ASGPR1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human ASGR1/ASGPR1 Fc Chimera (Catalog # 10255-AS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 59-71 kDa and 120-140 kDa, respectively.

Formulation, Preparation and Storage

10255-AS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASGR1/ASGPR1

The human asialoglycoprotein receptor (ASGPR) is an endocytic recycling receptor that belongs to the long-form subfamily of the C-type/Ca+2-dependent lectin family (1, 2, 3). It is a complex of two noncovalently-linked subunits, a major 46 kDa glycoprotein (ASGR1) and a minor 50 kDa glycoprotein (ASGR2). The major human ASGPR subunit, ASGR1 (also H1), is synthesized as a 291 amino acid (aa) type II transmembrane (TM) glycoprotein. It contains a 40 aa cytoplasmic region, a 21 aa TM segment, and a 230 aa extracellular domain (ECD) (4 - 6). The cytoplasmic region contains one palmitoylation site at Cys36 that is essential for ligand endocytosis and dissociation (7). The ECD contains two important structural regions. The first is a stalk region of 62 aa (aa 61-123) that contributes to noncovalent oligomerization. The second is a 118 aa, carbohydrate-binding, Ca+2-dependent C-type lectin domain (aa 161-278) that is stabilized by three Ca+2 ions (3, 8). Human ASGR1 ECD is 79% aa identical to mouse ASGR1 ECD. There are two minor (ASGR2) subunits that interact with ASGR1/H1 in a mutually exclusive manner to generate a functional ASGPR (9). They represent alternate splice forms of a type II TM protein. Termed H2b and H2c, H2b differs from H2c only by the presence of a 19 aa insert in its cytoplasmic region. This insert is significant because it allows serine phosphorylation of the cytoplasmic tail and provides for the majority of ASGPR ligand internalization (9). The stoichiometry of a functional ASGPR is unclear, but is suggested to be either a 2:2, 3:1 or 3:2 ratio of ASGR1/H1:ASGR2/H2 (9, 10, 11). ASGPR is found on hepatocytes and a subset of T cells (6, 12). ASGPR is reported to bind Gal (nonreducing), GalNAc, and sialic acid alpha2,6Gal and GalNAc (3, 13, 14, 15). This is generally within the context of triantennary or tetraantennary configurations (2). The sialic acid terminations are of particular interest because molecules with these motifs most likely represent the endogenous ligands for ASGPR (14).

References

  1. Stockert, R. J. (1995) Physiol. Rev. 75:591.
  2. Weigel, P.H. and J.H.N. Yik (2002) Biochim. Biophys. Acta 1572:341.
  3. Meier, M. et al. (2000) J. Mol. Biol. 300:857.
  4. Spiess, M. et al. (1985) J. Biol. Chem. 260:1979.
  5. Spiess, M. and H.F. Lodish (1986) Cell 44:177.
  6. Bischoff, J. et al. (1988) J. Cell Biol. 106:1067.
  7. Yik, J.H.N. et al. (2002) J. Biol. Chem. 277:40844.
  8. Monroe, R.S. and B.E. Huber (1994) Gene 148:237.
  9. Yik, J.H.N. et al. (2002) J. Biol. Chem. 277:23076.
  10. Bider, M.D. et al. (1996) J. Biol. Chem. 271:31996.
  11. Lodish, H. (1991) Trends Biochem. Sci. 16:374.
  12. Park, J-H. et al. (2006) Biotechnol. Lett. 28:1061.
  13. Westerlind, U. et al. (2004) Glyconj. J. 21:227.
  14. Park, E.I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17125.
  15. Park, E.I. et al. (2003) J. Biol. Chem. 278:4597.

Long Name

Asialoglycoprotein Receptor 1

Alternate Names

ASGPR1, CLEC4H1, MHL1, RHL1

Entrez Gene IDs

432 (Human); 11889 (Mouse); 24210 (Rat)

Gene Symbol

ASGR1

UniProt

Product Documents for Recombinant Human ASGR1/ASGPR1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human ASGR1/ASGPR1 Fc Chimera Protein, CF

For research use only

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