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Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein Best Seller

Catalog # 5860-AP | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
5860-AP-010/CF

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With Carrier
5860-AP-010

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human APRIL/TNFSF13 protein
HA
(YPYDVPDYA)
GCN4-IZ GGGSGGGSGGGS Human April
(Ala105-Leu250)
Accession # O75888
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

21.7 kDa

SDS-PAGE

26 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells.
The ED50 for this effect is 5-25 ng/mL in the presence of goat anti-mouse IgM.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 5860-AP
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 5860-AP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APRIL/TNFSF13

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily (1). It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule (2-4). Secreted human APRIL, which consists almost entirely of a single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL (2, 3). Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R (2, 5, 6). APRIL binds to heparan sulfate proteoglycans (HSPGs) independently of its binding to TACI and BCMA (6, 7). The interaction with HSPGs induces APRIL oligomerization, and this augments TACI-, or BMCA-mediated effects (7, 8). HSPGs are also critical for the tumor growth-promoting effects attributed to APRIL (6). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (10). TWE-PRIL is a bioactive hybrid protein produced by gene splicing. It consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (11). TWE-PRIL is expressed in monocytes and activated T cells and, in contrast to APRIL, is presented on the cell surface (11). APRIL enhances the proliferation and survival of plasma cells and also promotes T cell-dependent humoral responses (2, 12, 13). In the context of autoimmune disorders, however, APRIL can inhibit pathologic humoral responses as well as disease progression (14). Its expression by CD4+ T cells inhibits the production of Th2 cytokines and allergic inflammation (15). APRIL levels are elevated in the serum during coronary artery disease (16), and it is also elevated in many cancers primarily due to expression by tumor-infiltrating neutrophils (4, 7, 17).

References

  1. Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
  2. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  3. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
  4. Kelly, K. et al. (2000) Cancer Res. 60:1021.
  5. Day, E.S. et al. (2005) Biochemistry 44:1919.
  6. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
  7. Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
  8. Schwaller, J. et al. (2007) Blood 109:331.
  9. Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
  10. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  11. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.
  12. Benson, M.J. et al. (2008) J. Immunol. 180:3655.
  13. He, B. et al. (2004) J. Immunol. 172:3268.
  14. Fernandez, L. et al. (2012) Ann. Rheum. Dis. 72:1367.
  15. Xiao, Y. et al. (2011) Eur. J. Immunol. 41:164.
  16. Sandberg, W.J. et al. (2009) Thromb. Haemost. 102:704.
  17. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.

Long Name

A Proliferation-inducing Ligand

Alternate Names

CD256, TALL2, TNFSF13, TRDL1, ZTNF2

Entrez Gene IDs

8741 (Human); 69583 (Mouse); 287437 (Rat)

Gene Symbol

TNFSF13

UniProt

Product Documents for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

For research use only

Citations for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein (11)

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Customer Reviews for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein (12)

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  • Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
    Name: Gerre Lafond
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 02/19/2021
  • Name: Anthony Freeman
    Application: Cell migration/motility
    Verified Customer | Posted 12/02/2020
  • Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 10/15/2020
  • Name: Jenna Nguyen
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 02/08/2019
  • Name: Jenna Nguyen
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 01/30/2019
  • Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 01/30/2019
  • Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
    Name: Laura Howard
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/16/2019
    Soluble APRIL enhances NGF-promoted axon growth from SCG neurons. (A) Images of representative calcein-labelled newborn SCG neurons incubated for 16 h in medium containing 0.1 ng/ml NGF and 25 µM Boc-D-FMK with and without 100 ng/ml soluble recombinant APRIL. Scale bar:100 µm. (B) Bar charts of total length of axons in the arbors of P0 SCG neurons grown for 16 h in medium containing 0.1 ng/ml NGF, 25 µM Boc-D-FMK and different concentrations of APRIL.
    Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein 5860-AP
  • Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
    Name: Jenna Nguyen
    Application: Cell Adhesion
    Verified Customer | Posted 12/06/2018
  • Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
    Name: Jenna Nguyen
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/06/2018
  • Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
    Name: Jenna Nguyen
    Application: SDS-PAGE Control
    Verified Customer | Posted 11/05/2018
  • Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
    Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/05/2018
  • Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
    Name: Jenna Nguyen
    Application: Apoptosis assay
    Verified Customer | Posted 07/27/2018

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FAQs for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

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  • Q: What is the meaning of "HA (YPYDVPDYA)" in the Source section of the datasheet?

    A: HA (YPYDVPDYA) stands for Hemagglutinin, which consists of amino acids Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala. It is a popular epitope tag in proteins with mammalian expression systems.

  • Q: What is the structure of the Recombinant Human APRIL/TNFSF13 Protein (Catalog # 5860-AP)?

    A: We expect this Catalog # 5860-AP to be a non-covalently associated homotrimer as provided.

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