What is the meaning of "HA (YPYDVPDYA)" in the Source section of the datasheet?

Name: Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein
Brand: R&D Systems
SKU: 5860-AP
Rating: 5 (12 reviews)

HA (YPYDVPDYA) stands for Hemagglutinin, which consists of amino acids Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala. It is a popular epitope tag in proteins with mammalian expression systems.

Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 5860-AP

Citations (10)

(12)

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Key Product Details

Source:	HEK293
Accession #:	O75888
Applications:	Bioactivity

View Full Technical Details for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Product Specifications for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Source

Human embryonic kidney cell, HEK293-derived human APRIL/TNFSF13 protein

HA (YPYDVPDYA)	GCN4-IZ	GGGSGGGSGGGS	Human April (Ala105-Leu250) Accession # O75888
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Tyr

Predicted Molecular Mass

21.7 kDa

SDS-PAGE

26 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED₅₀ for this effect is 5-25 ng/mL in the presence of goat anti-mouse IgM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

5860-AP

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5860-AP/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APRIL/TNFSF13

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily (1). It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule (2-4). Secreted human APRIL, which consists almost entirely of a single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL (2, 3). Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R (2, 5, 6). APRIL binds to heparan sulfate proteoglycans (HSPGs) independently of its binding to TACI and BCMA (6, 7). The interaction with HSPGs induces APRIL oligomerization, and this augments TACI-, or BMCA-mediated effects (7, 8). HSPGs are also critical for the tumor growth-promoting effects attributed to APRIL (6). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (10). TWE-PRIL is a bioactive hybrid protein produced by gene splicing. It consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (11). TWE-PRIL is expressed in monocytes and activated T cells and, in contrast to APRIL, is presented on the cell surface (11). APRIL enhances the proliferation and survival of plasma cells and also promotes T cell-dependent humoral responses (2, 12, 13). In the context of autoimmune disorders, however, APRIL can inhibit pathologic humoral responses as well as disease progression (14). Its expression by CD4⁺ T cells inhibits the production of Th2 cytokines and allergic inflammation (15). APRIL levels are elevated in the serum during coronary artery disease (16), and it is also elevated in many cancers primarily due to expression by tumor-infiltrating neutrophils (4, 7, 17).

Item	Value
References	Planelles, L. et al. (2008) Curr. Mol. Med. 8:829. Yu, G. et al. (2000) Nat. Immunol. 1:252. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945. Kelly, K. et al. (2000) Cancer Res. 60:1021. Day, E.S. et al. (2005) Biochemistry 44:1919. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964. Hendriks, J. et al. (2005) Cell Death Differ. 12:637. Schwaller, J. et al. (2007) Blood 109:331. Ingold, K. et al. (2005) J. Exp. Med. 201:1375. Roschke, V. et al. (2002) J. Immunol. 169:4314. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711. Benson, M.J. et al. (2008) J. Immunol. 180:3655. He, B. et al. (2004) J. Immunol. 172:3268. Fernandez, L. et al. (2012) Ann. Rheum. Dis. 72:1367. Xiao, Y. et al. (2011) Eur. J. Immunol. 41:164. Sandberg, W.J. et al. (2009) Thromb. Haemost. 102:704. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
Long Name	A Proliferation-inducing Ligand
Entrez Gene IDs	8741 (Human); 69583 (Mouse); 287437 (Rat)
Alternate Names	A proliferation-inducing ligand, APRIL, APRILFLJ57090, CD256, CD256 antigen, TALL-2, TALL2, TNF- and APOL-related leukocyte expressed ligand 2, TNF-related death ligand 1, TNFSF13, TRDL-1, TRDL1, ZTNF2, tumor necrosis factor (ligand) superfamily, member 13, tumor necrosis factor ligand superfamily member 13, tumor necrosis factor-like protein ZTNF2, tumor necrosis factor-related death ligand-1

Citations for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein (10)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CC Chan et al. (2021), A BAFF/APRIL axis regulates obesogenic diet-driven weight gain Nature Communications, 12(1):2911.
PMID: 34006859
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Mulazzani et al. (2019), APRIL and BAFF: novel biomarkers for central nervous system lymphoma J Hematol Oncol, 12(1):102.
PMID: 31615554
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR

AW Clarke et al. (2018), An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 0(0):1-43.
PMID: 29436901
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

R Wilson et al. (2018), Condition-dependent generation of aquaporin-4 antibodies from circulating B cells in neuromyelitis optica Brain, 141(4):1063-1074.
PMID: 29447335
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

D Brach et al. (2017), EZH2 inhibition by tazemetostat results in altered dependency on B-cell activation signalingï¿½in DLBCL Mol. Cancer Ther., 0(0).
PMID: 28835384
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Saulep-Easton D et al. (2016), The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 30(1):163-72.
PMID: 26139429
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Mamara et al. (2015), TACI expression and signaling in chronic lymphocytic leukemia. J Immunol Res, 2015(0):478753.
PMID: 25950010
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tai Y et al. (2014), CRM1 inhibition induces tumor cell cytotoxicity and impairs osteoclastogenesis in multiple myeloma: molecular mechanisms and therapeutic implications. Leukemia, 28(1):155-65.
PMID: 23588715
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Deknuydt F et al. (2014), Diversion of the host humoral response: a novel virulence mechanism of Haemophilus influenzae mediated via outer membrane vesicles. J Leukoc Biol, 95(6):983-91.
PMID: 24550522
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Osorio C et al. (2014), Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci, 59(0):24-36.
PMID: 24444792

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Customer Reviews for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein (12)

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Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Name: Gerre Lafond

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 02/19/2021
Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Name: Anthony Freeman

Application: Cell migration/motility

Verified Customer | Posted 12/02/2020
Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Name: Anonymous

Application: Cell migration/motility

Verified Customer | Posted 10/15/2020
Name: Jenna Nguyen

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 02/08/2019
Name: Jenna Nguyen

Application: Enzymatic activity in vitro

Verified Customer | Posted 01/30/2019
Name: Jenna Nguyen

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 01/30/2019
Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Name: Laura Howard

Application: In vitro bioactivity in cell culture

Reason for Rating: Reliably promotes neurite outgrown of newborn mouse sympathetic neurons of the superior cervical ganglion.

Verified Customer | Posted 01/16/2019

Soluble APRIL enhances NGF-promoted axon growth from SCG neurons. (A) Images of representative calcein-labelled newborn SCG neurons incubated for 16 h in medium containing 0.1 ng/ml NGF and 25 µM Boc-D-FMK with and without 100 ng/ml soluble recombinant APRIL. Scale bar:100 µm. (B) Bar charts of total length of axons in the arbors of P0 SCG neurons grown for 16 h in medium containing 0.1 ng/ml NGF, 25 µM Boc-D-FMK and different concentrations of APRIL.
Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Name: Jenna Nguyen

Application: Cell Adhesion

Verified Customer | Posted 12/06/2018
Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Name: Jenna Nguyen

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 12/06/2018
Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Name: Jenna Nguyen

Application: SDS-PAGE Control

Verified Customer | Posted 11/05/2018
Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Name: Jenna Nguyen

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 11/05/2018
Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Name: Jenna Nguyen

Application: Apoptosis assay

Verified Customer | Posted 07/27/2018

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FAQs for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

What is the meaning of "HA (YPYDVPDYA)" in the Source section of the datasheet?

HA (YPYDVPDYA) stands for Hemagglutinin, which consists of amino acids Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala. It is a popular epitope tag in proteins with mammalian expression systems.

Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Formulation, Preparation and Storage

Carrier Free

5860-AP

Carrier

5860-AP/CF

Carrier Free

Reconstitution Calculator

Background: APRIL/TNFSF13

Citations for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein (10)

Customer Reviews for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein (12)

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Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

FAQs for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

Product Documents for Recombinant Human APRIL/TNFSF13 (HEK293-expressed) Protein

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