Recombinant Human APP/Protease Nexin II Protein, CF

Name: Recombinant Human APP/Protease Nexin II Protein, CF
Brand: R&D Systems
SKU: 3466-PI

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3466-PI

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	P05067
Applications:	Inhibition Activity

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Product Specifications for Recombinant Human APP/Protease Nexin II Protein, CF

Source	Mouse myeloma cell line, NS0-derived human APP protein Leu18-Leu688, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu18
Predicted Molecular Mass	77 kDa
SDS-PAGE	120 kDa, reducing conditions
Activity	Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (Catalog # ES002). The IC₅₀ value is <1.2 nM, as measured with under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3466-PI

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile, deionized water. Reconstitution Buffer Available: Size / Price Qty Loading... RB07:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: APP

Amyloid precursor protein (APP) is a type I membrane protein with several human isoforms due to alternative splicing. APP-770, -751, and -733 contain a Kunitz protease inhibitor (KPI) domain (residue 291-342) and APP-695 does not. APP is a cell surface molecule with many functions. It can be processed proteolytically in two different pathways. In one pathway, beta - and gamma -secretase cleave at the beta site between residue 670 and 671 and the gamma site between residue 711 and 714 to produce beta ‑amyloid peptide (A beta 40 and A beta 42), a major component in plaques found in brains of patients with Alzheimer's disease (1). The other pathway involves alpha -secretase that cleaves residues between 687 and 688. It is antiamyloidogenic due to its benign character and the prevention of the A beta peptide formation (2). Soluble APP containing the KPI domain, also referred to as protease nexin II, is a potent inhibitor of serine proteases and may have additional functions. For example, it may regulate the contact face of blood coagulation and limit thrombosis specially in brain due to its localization and coagulation factor XI inhibiting activity (3, 4).

Item	Value
References	Haass, C. (2004) EMBO J. 23:483. Lichtenthaler, S. F. and C. Haass (2004) J. Clin. Invest. 113:1384. Badellino, K.O. and P.N. Walsh (2000) Biochemistry 39:4769. Xu, F. et al. (2005) Proc. Natl. Acad. Sci USA. 102:18135.
Long Name	Amyloid Precursor Protein
Entrez Gene IDs	351 (Human); 11820 (Mouse)
Alternate Names	APP, Amyloid beta, Amyloid beta precursor protein, Protease Nexin II, amyloid beta (A4) precursor protein-binding, family B, member 2, amyloid beta A4 precursor protein-binding family B member 2, beta Amyloid

Citations for Recombinant Human APP/Protease Nexin II Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human APP/Protease Nexin II Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Thomas RS et al. (2011), Anti-amyloid precursor protein immunoglobulins inhibit amyloid-beta production by steric hindrance. FEBS J., 278(1):167-78.
PMID: 21122073
Species: N/A
Sample Types: N/A
Applications: Enzyme Assay Substrate

Ahmed RR et al. (2010), BACE1 and BACE2 enzymatic activities in Alzheimer's disease. J. Neurochem., 112(4):1045-53.
PMID: 19968762

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