Recombinant Human ANGPTL8/Betatrophin His-tag Protein, CF

Name: Recombinant Human ANGPTL8/Betatrophin His-tag Protein, CF
Brand: rnd
SKU: 10159-AN

Catalog # 10159-AN

Datasheet / COA / SDS

Mucin Stalk Chimera

Order Information

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Key Product Details

Source:	CHO
Accession #:	Q6UXH0 (Angiopoietin-like Protein 8/Betatrophin) &
Applications:	Bioactivity

View Full Technical Details for Recombinant Human ANGPTL8/Betatrophin His-tag Protein, CF

Technical Data
Citations / Reviews / FAQs

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 8/Betatrophin protein

Human Angiopoietin-like Protein 8/Betatrophin (Ala22-Ala198) Accession # Q6UXH0	Human Fractalkine mucin-like stalk (Phe103-Thr338) Accession # P78423	6-His tag
N-terminus		C-terminus

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala22

Predicted Molecular Mass

45 kDa

SDS-PAGE

70-93 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin-like Protein 3/ANGPTL3 (Catalog # 3829-AN) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human Angiopoietin-like Protein 8/Betatrophin that produces 50% of the optimal binding response is 0.15‑1.2 μg/mL.

Recombinant Human ANGPTL8/Betatrophin His-tag Protein, CF Scientific Data Examples

	Recombinant Human ANGPTL8/Betatrophin His-tag Protein Binding Activity When Recombinant Human Angiopoietin-like Protein 3/ANGPTL3 (Catalog # 3829-AN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human ANGPTL8/Betatrophin (Catalog # 10159-AN) binds with an ED₅₀of 0.15-1.2 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10159-AN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and CHAPS with Trehalose.
Reconstitution	Reconstitute at 400 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Angiopoietin-like Protein 8/Betatrophin

Betatrophin (also called ANGPTL8, lipasin and RIPL), a novel secretory protein from liver and fatty tissues, is believed to be involved in lipid and glucose metabolism. It is most homologous to ANGPTL3 but lacks the fibrinogen-like domain of ANGPTL3 and other ANGPTL family members (1). Human Betatrophin is 183 amino acids (aa) in length and shares 72% aa identity with mouse Betatrophin. Betatrophin is expressed in the liver, white adipose, and brown adipose tissues. It is a recently identified hormone which has been associated with two functionally important processes in the development of type 2 diabetes and obesity, insulin resistance as well as being a crucial modulator in lipid metabolism through regulation of serum triglyceride levels (2, 3). Betatrophin can form a complex with the N-terminal of ANGPTL3, and this complex is necessary for inhibition of lipoprotein lipase (LPL) activity and triglyceride modulation (4). Elevated levels of circulating betatrophin in the blood may be associated with gestational diabetes mellitus (GDM) and polycystic ovary syndrome (PCOS) (5, 6). Increased levels of Betatrophin in plasma and adipose tissue may also be associated with hypertension, coronary artery disease (CAD), and peripheral artery disease (PAD) (7, 8). The role of Betatrophin in inducing pancreatic beta-cell proliferation is controversial, as it did not control beta-cell expansion in the mouse model (9, 10). In addition to the lipid and glucose metabolism, ANGPTL8 has been reported to be involved in many other disorders such as non-alcoholic fatty liver disease and renal dysfunction (11).

Item	Value
References	Quagliarini, F. et al. (2012) PNAS 109:19751. Abu-Farha M. et al. (2017) Diabetes Metab Res Rev. 33:e2919. Zhang R. et al. (2012) Biochem Biophys Res Commun. 424:786 Chi, X. et al. (2017) Molecular Metabolism 6:1137. Kong F.J. et al. (2017) PLoS One. 12:e0169941. Varikasuvu S.R. et al. (2019) Gynecol Endocrinol. 35:190 Abu-Farha M. et al. (2018) Lipids Health Dis. 17:35 Niki H. et al. (2019) J Atheroscler Thromb. 26:573. Abu-Farha M. et al. (2016) J Diabetes Res. 2016:4860595. Cox A.R. et al. PLoS One (2016) 11:e0159276. Luo, M. et al. (2018) Front Endocrino 9:169.
Entrez Gene IDs	55908 (Human); 624219 (Mouse); 100361444 (Rat)
Alternate Names	ANGPTL8, Angiopoietin like Protein 8, Angiopoietin-like Protein 8, Betatrophin, C19orf80, Gm6484, Lipasin, PRO1185, PVPA599, RIFL, TD26, UNQ599, chromosome 19 open reading frame 80, hepatocellular carcinoma-associated gene TD26, hepatocellular carcinoma-associated protein TD26

Citations

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