Recombinant Human alpha-L-Iduronidase/IDUA Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4119-GH
NS0-expressed
Key Product Details
- R&D Systems NS0-derived Recombinant Human alpha-L-Iduronidase/IDUA Protein (4119-GH)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human alpha-L-Iduronidase/IDUA protein
Ala26-Pro653 (Ala26Thr), with a C-terminal 10-His tag
Ala26-Pro653 (Ala26Thr), with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr26
Predicted Molecular Mass
71 kDa
SDS-PAGE
83 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl alpha-L-iduronide.
The specific activity is >7,500 pmol/min/µg, as measured under the described conditions.
The specific activity is >7,500 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 4119-GH in the following applications:
Scientific Data Images for Recombinant Human alpha-L-Iduronidase/IDUA Protein, CF
Recombinant Human alpha‑L‑Iduronidase/IDUA Protein SEC-MALS.
Recombinant Human alpha‑L‑Iduronidase/IDUA Protein (Catalog # 4119-GH) has a molecular weight (MW) of 99-110 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human alpha-L-Iduronidase/IDUA Protein Activity Assay Diagram.
Recombinant Human alpha-L-Iduronidase/IDUA Protein, CF (Catalog # 4119-GH) hydrolyzes iduronic acid from dermatan and heparan sulfates.
Recombinant Human alpha-L-Iduronidase/IDUA Protein Activity Assay.
Recombinant Human alpha-L-Iduronidase/IDUA Protein, CF (Catalog # 4119-GH) is measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl alpha -L-iduronide.Formulation, Preparation and Storage
4119-GH
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: alpha-L-Iduronidase/IDUA
References
- Scott, H.S. et al. (1995) Hum. Mutat. 6:288.
- Wraith, J.E. (2005) Expert Opin. Pharmacother. 6:489.
Alternate Names
alphaLIduronidase, IDA, IDUA
Gene Symbol
IDUA
UniProt
Additional alpha-L-Iduronidase/IDUA Products
Product Documents for Recombinant Human alpha-L-Iduronidase/IDUA Protein, CF
Product Specific Notices for Recombinant Human alpha-L-Iduronidase/IDUA Protein, CF
For research use only
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