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Recombinant Human alpha-L-Iduronidase/IDUA His Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11180-GH

CHO-expressed (aa 26-653)
Catalog #
Size / Price

Key Product Details



Accession #




Enzyme Activity

Product Specifications


Chinese Hamster Ovary cell line, CHO-derived human alpha-L-Iduronidase/IDUA protein
Ala26-Pro653, with a C-terminal 10-His tag


>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

71 kDa


80-88 kDa, under reducing conditions.


Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl  alpha-L-iduronide.
The specific activity is >17,000 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human alpha-L-Iduronidase/IDUA His Protein, CF

Recombinant Human alpha-L-Iduronidase/IDUA His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human alpha-L-Iduronidase/IDUA His-tag Protein (Catalog # 11180-GH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-88 kDa.

Formulation, Preparation and Storage

Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: alpha-L-Iduronidase/IDUA

a-L-Iduronidase is a member of the glycoside hydrolase family encoded by the IDUA gene (1). It is an important enzyme required for the lysosomal degradation of glycosaminoglycans (GAGS) and hydrolyzes the non-reducing terminal a-L-iduronic acid residues in GAGS including dermatan sulfate and heparan sulfate.  Human IDUA is a 653 aa protein composed of a signal peptide removed in the lysosome for mature form and three domains: a triosephosphate isomerase barrel fold containing the catalytic site, a B-sandwich domain, and an Ig(Ig)-like domain. The protein has six reported N-glycosylation sites and the glycosylation status of the enzyme correlates with its catalytic activity (1). More than 55-disease associated missense mutations in the IDUA gene have been identified (1). Mutations in IDUA that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I) (2). MPS I can be classified as three clinical subtypes; Hurler syndrome, Hurler-Scheie syndrome, and Scheie syndrome with decreasing severity, respectively. MPS I causes progressive cellular, tissue and organ damage, and several clinical studies using enzyme replacement therapy show positive results (3,4). More recently, the IDUA gene has been linked to osteoporosis (5,6).


  1. Maita, N. et al. (2013) Proc. Natl. Acad. Sci. 110:14628.
  2. Scott, H.S. et al. (1995) Hum. Mutat. 6:288.
  3. Wraith, J.E. (2005) Expert Opin. Pharmacother. 6:489.
  4. Jameson, E. (2016) Cochrane Database Syst. Rev. 4: CD009354.
  5. Kodric, K. et al. (2016) Wien Klin Wochenschr. 128:480.
  6. Niu, T. et al. (2016) J. Bone Miner. Res. 31:358.

Alternate Names

alphaLIduronidase, IDA, IDUA

Entrez Gene IDs

3425 (Human); 15932 (Mouse); 360904 (Rat); 102116889 (Cynomolgus Monkey)

Gene Symbol



Additional alpha-L-Iduronidase/IDUA Products

Product Documents for Recombinant Human alpha-L-Iduronidase/IDUA His Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human alpha-L-Iduronidase/IDUA His Protein, CF

For research use only