Is ACE-1 the same as ACE?

Name: Recombinant Human ACE Protein, CF
Brand: R&D Systems
SKU: 929-ZN
Rating: 5 (1 reviews)

Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.

Recombinant Human ACE Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 929-ZN

Citations (6)

(1)

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Key Product Details

Source:	NS0
Accession #:	P12821.1
Structure / Form:	Recombinant Human ACE/CD143 Somatic Form is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human ACE Protein, CF

Source	Mouse myeloma cell line, NS0-derived human ACE/CD143 protein Leu30-Leu1261, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu30
Predicted Molecular Mass	143 kDa
SDS-PAGE	160-180 kDa, reducing conditions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005). The specific activity is >1,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

929-ZN

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl₂ and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: ACE/CD143

ACE (also known as peptidyl-dipetidase A) is a zinc metallopeptidase important for blood pressure control and water and salt metabolism (2). It cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II and inactivates bradykinin by the sequential removal of two C-terminal dipeptides. In addition to the two physiological substrates, ACE cleaves C-terminal dipeptides from various oligopeptides with a free C-terminus. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. For example, ACE degrades Alzheimer amyloid beta -peptide (A beta ), retards A beta aggregation, deposition, fibril formation, and inhibits cytotoxicity (3).

ACE is a type I membrane protein and exists in two isoforms (2). Somatic ACE, found in endothelial, epithelial and neuronal cells, comprises two highly similar domains called N- and C-domains, each of which contains the HExxH consensus sequence for zinc binding. Germinal ACE, found exclusively in the testes, comprises a single catalytically active domain identical to the C-domain of somatic ACE except for an N-terminal 67 residue germinal ACE-specific sequence. Physiological functions of the two tissue-specific isozymes are not interchangeable (4). For example, sperm-specific expression of the germinal ACE, not the somatic ACE, in ACE knockout male mice restored fertility.

Soluble ACE is present in many biological fluids, such as serum, seminal fluid, amniotic fluid and cerebrospinal fluid (2). The soluble ACE is derived from the membrane forms by actions of secretases or sheddases. The identities of the secretases have not been revealed, although they belong to the family of zinc metallopeptidases (5, 6).

Item	Value
References	Soubrier, et al. (1988) Proc. Natl. Acad. Sci. USA 85:9386. Corvol, P. and T.A. Williams (1998) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds): San Diego, Academic Press, p. 1066. Hu, et al. (2001) J. Biol. Chem. 276:47863. Kessler, et al. (2000) J. Biol. Chem. 275:26259. Eyries, et al. (2001) J. Biol. Chem. 276:5525. Alfalah, et al. (2001) J. Biol. Chem. 276:21105.
Long Name	Angiotensin I Converting Enzyme
Entrez Gene IDs	1636 (Human); 11421 (Mouse)
Alternate Names	ACE, ACE1angiotensin converting enzyme, somatic isoform, CD143, CD143 antigen, DCP, DCP1, DCP1angiotensin-converting enzyme, Dipeptidyl carboxypeptidase I, EC 3.2.1.-, EC 3.4.15.1, Kininase II, MGC26566, MVCD3, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1, carboxycathepsin, dipeptidyl carboxypeptidase 1, peptidase P, testicular ECA

Citations for Recombinant Human ACE Protein, CF (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human ACE Protein, CF include:

Species: Human
Sample Types: Protein
Applications: Bioassay

NS Al Mulhim et al. (2019), Divergence in the activity of the N- and C- catalytic domains of ACE1 - implications for the role of the renin-angiotensin system in Alzheimer's disease Acta Neuropathol Commun, 7(1):57.
PMID: 31018864
Species: Human
Sample Types: Urine
Applications: Bioassay

AM South et al. (2018), Obesity is Associated with Higher Blood Pressure and Higher Levels of Angiotensin II but Lower Angiotensin-(1-7) in Adolescents Born Preterm J. Pediatr., 0(0).
PMID: 30404738
Species: Human
Sample Types: Protein
Applications: Bioassay

Fagyas M et al. (2014), New perspectives in the renin-angiotensin-aldosterone system (RAAS) II: albumin suppresses angiotensin converting enzyme (ACE) activity in human. PLoS ONE, 9(4):e87844.
PMID: 24691203
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Roosterman D et al. (2008), Endothelin-converting enzyme-1 degrades internalized somatostatin-14. Endocrinology, 149(5):2200-7.
PMID: 18276747
Species: Human
Sample Types: Peptide
Applications: Enzyme Assay

Miners JS et al. (2008), Immunocapture-based fluorometric assay for the measurement of neprilysin-specific enzyme activity in brain tissue homogenates and cerebrospinal fluid. J. Neurosci. Methods, 167(2):229-36.
PMID: 17904641
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Bioassay

de Lang A et al. (2006), Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells. Virology, 353(2):474-81.
PMID: 16860835

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Name: Shrinidh Joshi

Verified Customer | Posted 08/04/2016

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FAQs for Recombinant Human ACE Protein, CF

Is ACE-1 the same as ACE?

Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.

Recombinant Human ACE Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human ACE Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: ACE/CD143

Citations for Recombinant Human ACE Protein, CF (6)

Customer Reviews for Recombinant Human ACE Protein, CF (1)

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Recombinant Human ACE Protein, CF

FAQs for Recombinant Human ACE Protein, CF

Product Documents for Recombinant Human ACE Protein, CF

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