Recombinant E. coli Methionine Aminopeptidase/METAP, CF
R&D Systems, part of Bio-Techne | Catalog # 1628-ZN
Key Product Details
- R&D Systems E. coli-derived Recombinant E. coli Methionine Aminopeptidase/METAP (1628-ZN)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
E. coli-derived e. coli Methionine Aminopeptidase protein
Ala2-Glu264, with a C-terminal 10-His tag
Ala2-Glu264, with a C-terminal 10-His tag
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala2
Predicted Molecular Mass
31 kDa
SDS-PAGE
36 kDa, reducing conditions
Activity
Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 9168-SE).
The specific activity is >0.5 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
The specific activity is >0.5 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
Formulation, Preparation and Storage
1628-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Methionine Aminopeptidase
Methionine Aminopeptidase (METAP) carries out the important reaction of removing the initiator Met residue from nascent proteins. There are two types of METAP (1, 2). Type Ia and type IIa are found in eubacteria and archaea, respectively. Type Ib and type IIb are present in eukaryotes and contain additional N-terminal domains. As a metalloenzyme, METAP activity can be inhibited by EDTA. However, the exact identity of the metal ion remains to be uncovered since several metal ions including cobalt, zinc and iron have been suggested (3).
References
- Bradshaw, R.A. et al. (1998) Trends Biochem. Sci. 23:263.
- Lowether, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta 1477:157.
- Li, J-Y. et al. (2003) Biochem. Biophys. Res. Comm. 307:172.
Entrez Gene IDs
947882 (E. coli)
Gene Symbol
MAP
UniProt
Additional Methionine Aminopeptidase Products
Product Documents for Recombinant E. coli Methionine Aminopeptidase/METAP, CF
Product Specific Notices for Recombinant E. coli Methionine Aminopeptidase/METAP, CF
For research use only
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