Recombinant Cynomolgus SIRP beta 1/CD172b Fc Protein, CF

Name: Recombinant Cynomolgus SIRP beta 1/CD172b Fc Protein, CF
Brand: R&D Systems
SKU: 10312-SB

Catalog # 10312-SB

Product Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	XP_005568593.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus SIRP beta 1/CD172b Fc Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey SIRP beta 1/CD172b protein

Cynomolgus Monkey SIRP beta 1/CD172b (Glu30-Pro369) Accession # XP_005568593.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu30

Predicted Molecular Mass

64 kDa

SDS-PAGE

88-99 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey SIRP beta 1/CD172b Fc Chimera (Catalog # 10312-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP‑D (Catalog # 1920-SP) binds with an ED₅₀ of 40-320 ng/mL.

Recombinant Cynomolgus SIRP beta 1/CD172b Fc Protein, CF Scientific Data Examples

	Recombinant Cynomolgus SIRP beta 1/CD172b Fc Protein Binding Activity When Recombinant Cynomolgus Monkey SIRP beta 1/CD172b Fc Chimera (Catalog # 10312-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP-D (Catalog # 1920-SP) binds with an ED₅₀ of 40-320 ng/mL.
	Recombinant Cynomolgus SIRP beta 1/CD172b Fc Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey SIRP beta 1/CD172b Fc Chimera (Catalog # 10312-SB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 88-99 kDa and 170-200 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10312-SB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: SIRP beta 1/CD172b

Signal-regulatory protein beta 1 (SIRP beta 1) is a disulfide-linked type I membrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily (1). Mature cynomolgus SIRP beta 1 consists of a 342 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a short 4 aa cytoplasmic domain. Within the ECD, cynomolgus SIRP beta 1 shares 87% aa sequence identity with human SIRP beta 1. The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains (2). Positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs) (3). Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages (4). SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induce tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation (5, 6). Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma receptor-dependent or -independent phagocytosis in mouse peritoneal macrophages (7). Surfactant protein D (SP-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47 (8). Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47 (9).

Item	Value
References	vanBeek, E.M. et al. (2005) J. Immunol. 175:7781. van den Berg, T. et al. (2008) Trends in Immunology 29:203. Liu, Y. et al. (2005) Journal of Biological Chemistry 280:36132. Matozaki, T. et al. (2009) Trends in Cell Biology 19:72. Dietrich, J. et al. (2000) J Immunol. 164:9. Brook, G. et al. (2004) J Immunol. 173:2562. Hayashi, A. et al. (2004) J Biol Chem. 279:29450. Fournier, B. et al. (2012) J. Biol. Chem. 287:19386. Seiffert, M. et al. (2001) Blood 97:2741.
Long Name	Signal-regulatory Protein beta 1
Entrez Gene IDs	10326 (Human); 102139733 (Cynomolgus Monkey)
Alternate Names	CD172 antigen-like family member B, CD172b, CD172b antigen, DKFZp686A05192, FLJ26614, SIRP beta 1, SIRP-BETA-1, SIRP-beta-1 isoform 3, SIRPB1, signal-regulatory protein beta 1, signal-regulatory protein beta-1

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