Chinese Hamster Ovary cell line, CHO-derived Dkk-1 protein Thr32-His266
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
35-39 kDa, under reducing conditions
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 12.5-100 ng/mL.
Recombinant Cynomolgus/Rhesus Macaque Dkk-1 Protein, CF Scientific Data Examples
Recombinant Cynomolgus/Rhesus Macaque Dkk-1 Protein Bioactivity
Recombinant Cynomolgus Monkey/Rhesus Macaque Dkk-1 (10450-DK) inhibits Recombinant Mouse Wnt-3a (1324-WN) induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 12.5-100 ng/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature Cynomolgus/rhesus Dkk-1 is a 40 kDa glycosylated protein that shares 99% amino acid sequence identity with human Dkk-1. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). The C-terminal cysteine-rich domain 2 (CRD2) of Dkk-1 has been shown to interact with LRP5 or LRP6 (5). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 6). Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 6). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (7, 8). Other sites of expression include developing neurons, hair follicles and the retina of the eye (9, 10). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (11). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (9, 12). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (13).
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Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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