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Recombinant Cynomolgus OX40/TNFRSF4 Fc Chimera Protein, CF

Catalog # 10311-OX | R&D Systems, Inc. a Bio-Techne Brand
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10311-OX-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey OX40/TNFRSF4 protein
Cynomolgus Monkey OX40/TNFRSF4
(Leu29-Ala216)
Accession # XP_005545179
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

47 kDa

SDS-PAGE

62-72 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) is immobilized at 250 ng/mL, the concentration of Recombinant Cynomolgus Monkey OX40/TNFRSF4 Fc Chimera (Catalog# 10311-OX) that produces 50% optimal binding response is found to be approximately 5-30 ng/mL.

Scientific Data Images for Recombinant Cynomolgus OX40/TNFRSF4 Fc Chimera Protein, CF

Recombinant Cynomolgus OX40/TNFRSF4 Fc Chimera Protein Binding Activity

Recombinant Cynomolgus OX40/TNFRSF4 Fc Chimera Protein Binding Activity

When Recombinant Human OX40 Ligand/TNFSF4 Protein (Catalog # 1054-OX) is immobilized at 250 ng/mL, the concentration of Recombinant Cynomolgus Monkey OX40/TNFRSF4 Fc Chimera (Catalog # 10311-OX) that produces the 50% optimal binding response is found to be approximately 5-30 ng/mL.
Recombinant Cynomolgus OX40/TNFRSF4 Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus OX40/TNFRSF4 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey OX40/TNFRSF4 Fc Chimera (Catalog # 10311-OX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-72 kDa and 125-145 kDa, respectively.

Formulation, Preparation and Storage

10311-OX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OX40

OX40 (CD134; TNFRSF4) is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response (1-3). Cynomolgous OX40 is a 47 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 187 aa extracellular domain (ECD) that contains a cysteine-rich region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (4). The ECD of Cynomolgous OX40 shares 95%, 65% and 64% amino acid sequence identity with the ECD of human, mouse and rat OX40, respectively. OX40 is up‑regulated on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7 (5, 6). OX40 Ligand is primarily expressed on antigen presenting cells (5). OX40 Ligand engagement of OX40 on activated CD4+ T cells results in increased T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (T regs) and can promote the maintenance of and recall response in memory T cells (3, 7-10). OX40 is constitutively expressed on Tregs and enhances the sensitivity of T regs to IL-2, thus promoting Treg proliferation. OX40 has also been shown to decrease the cells' immunosuppressive activity on effector T cells (11-14). OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease and autoimmune disease (6, 15, 16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (17, 18).

References

  1. Hori, T. (2006) Int. J. Hematol. 83:17.
  2. Latza, U. et al. (1994) Eur. J. Immunol. 24:677.
  3. Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315.
  4. al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695.
  5. Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230.
  6. Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
  7. Xiao, X. et al. (2008) J. Immunol. 181:3193.
  8. So, T. and M. Croft (2007) J. Immunol. 179:1427.
  9. Mousavi, S.F. et al. (2008) J. Immunol. 181:5990.
  10. Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
  11. Piconese, S. et al. (2010) Eur. J. Immunol. 40:2902.
  12. Griseri, T. et al. (2010) J. Exp. Med. 207:699.
  13. Xiao, X. et al. (2012) J. Immunol. 188:892.
  14. Vu, M.D. et al. (2007) Blood 110:2501.
  15. Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688.
  16. Xiao, X. et al. (2012) Nat. Immunol. 13:981.
  17. Nakano, M. et al. (2010) Cardiovasc. Res. 88:539.
  18. Ishii, N. et al. (2010) Adv. Immunol. 105:63.
  19. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757.

Alternate Names

ACT-135, CD134, Ly-70, TNFRSF4, Txgp1, TXGP1L

Entrez Gene IDs

7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)

Gene Symbol

TNFRSF4

UniProt

Product Documents for Recombinant Cynomolgus OX40/TNFRSF4 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus OX40/TNFRSF4 Fc Chimera Protein, CF

For research use only

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